- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
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产品名称Anti-Pleiotrophin抗体
参阅全部 Pleiotrophin 一抗 -
描述兔多克隆抗体to Pleiotrophin
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经测试应用适用于: ICC/IF, IHC-Pmore details
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种属反应性与反应: Chicken, Human
预测可用于: Mouse, Rat, Cow, Dog, Pig
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Pleiotrophin.
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阳性对照
- This antibody gave a positive signal in human hippocampus formalin fixed paraffin embedded tissue section.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab95391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能Heparin binding mitogenic protein. Has neurite extension activity.
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组织特异性Osteoblast and brain.
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序列相似性Belongs to the pleiotrophin family.
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细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 280904 Cow
- Entrez Gene: 5764 Human
- Entrez Gene: 19242 Mouse
- Entrez Gene: 397609 Pig
- Entrez Gene: 24924 Rat
- Omim: 162095 Human
- SwissProt: P32760 Chicken
- SwissProt: P21782 Cow
see all -
别名
- HARP antibody
- HB-GAM antibody
- HBBM antibody
see all
Anti-Pleiotrophin antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pleiotrophin antibody (ab95391)IHC image of Pleiotrophin staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab95391, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunocytochemistry/ Immunofluorescence - Pleiotrophin antibody (ab95391)ICC/IF image of ab95391 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab95391, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Pleiotrophin antibody (ab95391)参考文献
This product has been referenced in:
- Zhu X et al. The effects of pleiotrophin in proliferative diabetic retinopathy. PLoS One 10:e0115523 (2015). IHC-FoFr, ICC/IF ; Human . Read more (PubMed: 25617851) »
- Lee JY et al. Chicken pleiotrophin: regulation of tissue specific expression by estrogen in the oviduct and distinct expression pattern in the ovarian carcinomas. PLoS One 7:e34215 (2012). IHC-P ; Chicken . Read more (PubMed: 22496782) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"