概述

  • 产品名称Anti-Pleiotrophin抗体
    参阅全部 Pleiotrophin 一抗
  • 描述
    兔多克隆抗体to Pleiotrophin
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Chicken, Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Pleiotrophin.

  • 阳性对照
    • This antibody gave a positive signal in human hippocampus formalin fixed paraffin embedded tissue section.

性能

应用

Our Abpromise guarantee covers the use of ab95391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Heparin binding mitogenic protein. Has neurite extension activity.
  • 组织特异性Osteoblast and brain.
  • 序列相似性Belongs to the pleiotrophin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HARP antibody
    • HB-GAM antibody
    • HBBM antibody
    • HBGAM antibody
    • HBGF-8 antibody
    • HBGF8 antibody
    • HBNF antibody
    • HBNF-1 antibody
    • HBNF1 antibody
    • heparin affin regulatory protein antibody
    • Heparin binding growth associated molecule antibody
    • Heparin binding growth factor 8 antibody
    • Heparin binding neurite outgrowth promoting factor 1 antibody
    • Heparin-binding brain mitogen antibody
    • Heparin-binding growth factor 8 antibody
    • Heparin-binding growth-associated molecule antibody
    • heparin-binding neurite outgrowth promoting factor antibody
    • Heparin-binding neurite outgrowth-promoting factor 1 antibody
    • NEGF1 antibody
    • Neurite growth promoting factor 1 antibody
    • Neurite outgrowth-promoting factor, heparin-binding antibody
    • OSF-1 antibody
    • OSF1 antibody
    • Osteoblast-specific factor 1 antibody
    • pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) antibody
    • Pleiotrophin antibody
    • PTN antibody
    • PTN_HUMAN antibody
    see all

Anti-Pleiotrophin antibody 图像

  • IHC image of Pleiotrophin staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab95391, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ICC/IF image of ab95391 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab95391, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Pleiotrophin antibody (ab95391)参考文献

This product has been referenced in:
  • Zhu X  et al. The effects of pleiotrophin in proliferative diabetic retinopathy. PLoS One 10:e0115523 (2015). IHC-FoFr, ICC/IF ; Human . Read more (PubMed: 25617851) »
  • Lee JY  et al. Chicken pleiotrophin: regulation of tissue specific expression by estrogen in the oviduct and distinct expression pattern in the ovarian carcinomas. PLoS One 7:e34215 (2012). IHC-P ; Chicken . Read more (PubMed: 22496782) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab95391.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"