概述

  • 产品名称Anti-Plectin抗体[E398P]
    参阅全部 Plectin 一抗
  • 描述
    兔单克隆抗体[E398P] to Plectin
  • 特异性ab32528 recognises plectin, a 500-kDa intermediate filament binding protein.
  • 经测试应用适用于: WB, IHC-P, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A recombinant fragment comprising the C-terminal 220 amino acids of plectin.

  • 阳性对照
    • Jurkat cell lysate, human skeletal muscle
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab32528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 500 kDa (predicted molecular weight: 531 kDa).
IHC-P 1/250.
IP 1/40.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.
    • 组织特异性Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
    • 疾病相关Epidermolysis bullosa simplex with pyloric atresia
      Epidermolysis bullosa simplex, with muscular dystrophy
      Epidermolysis bullosa simplex, Ogna type
      Limb-girdle muscular dystrophy 2Q
    • 序列相似性Belongs to the plakin or cytolinker family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 33 plectin repeats.
      Contains 4 spectrin repeats.
    • 结构域The N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N-and the C-terminus can bind integrin beta-4.
    • 翻译后修饰Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
    • 细胞定位Cytoplasm > cytoskeleton. Cell junction > hemidesmosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EBS1 antibody
      • EBSO antibody
      • HD1 antibody
      • Hemidesmosomal protein 1 antibody
      • PCN antibody
      • pleC antibody
      • PLEC_HUMAN antibody
      • PLEC1 antibody
      • PLEC1b antibody
      • Plectin 1 antibody
      • Plectin 1 intermediate filament binding protein 500kDa antibody
      • Plectin 6 antibody
      • Plectin antibody
      • Plectin-1 antibody
      • PLTN antibody
      see all

    Anti-Plectin antibody [E398P] 图像

    • Immunohistochemical staining of paraffin-embedded human skeletal muscle using ab32528 at a dilution of 1/250
    • Fluorescent immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab32528. Green-Plectin red-PI

    Anti-Plectin antibody [E398P] (ab32528)参考文献

    This product has been referenced in:
    • Kulkarni RM  et al. Ron receptor signaling is protective against DSS-induced colitis in mice. Am J Physiol Gastrointest Liver Physiol 306:G1065-74 (2014). Mouse . Read more (PubMed: 24742989) »
    • Eisenberg JL  et al. Plectin-containing, centrally localized focal adhesions exert traction forces in primary lung epithelial cells. J Cell Sci 126:3746-55 (2013). ICC/IF ; Human . Read more (PubMed: 23750011) »

    See all 12 Publications for this product

    Product Wall

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Rabbit monoclonal [E398P] to Plectin (ab32528) is yet to be tested for use by immunocytochemistry and therefore we cannot guarantee it for thi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"