概述

  • 产品名称Anti-PLD3抗体
    参阅全部 PLD3 一抗
  • 描述
    兔多克隆抗体to PLD3
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A synthetic peptide corresponding to a region of Human PLD3

  • 阳性对照
    • Fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab76433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:62500.

靶标

Anti-PLD3 antibody 图像



  • Predicted band size : 55 kDa
    Western blot analysis of Human adult placenta tissue lysate labeling PLD3 with ab76433 at 1.0µg/ml.
  • Anti-PLD3 antibody (ab76433) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Gel concentration 12%

Anti-PLD3 antibody (ab76433)参考文献

ab76433 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Zebrafish Tissue lysate - whole (total larvae, 4 days post fertilization)
Specification total larvae, 4 days post fertilization
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Evgenia Salta

Verified customer

提交于 Oct 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"