RabMAb

Anti-PLCG 2 (phospho Y1217 + Y1217)抗体[EP1404Y] (ab52610)

概述

  • 产品名称Anti-PLCG 2 (phospho Y1217 + Y1217)抗体[EP1404Y]
    参阅全部 PLCG 2 一抗
  • 描述
    兔单克隆抗体[EP1404Y] to PLCG 2 (phospho Y1217 + Y1217)
  • 特异性ab52610 only detects PLCG2 phosphorylated on Tyrosine 1217.
  • 经测试应用适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC/IF
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PLCG 2 aa 1200 to the C-terminus (phospho Y1217).

  • 阳性对照
    • Raji cell lysate; human lymph node tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab52610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
IP 1/50.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt or ICC/IF.
  • 靶标

    • 功能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
    • 序列相似性Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • 翻译后修饰Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase gamma-2 antibody
      • EC 3.1.4.11 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-2 antibody
      • Phospholipase C gamma 2 antibody
      • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
      • Phospholipase C-gamma-2 antibody
      • Phospholipase C-IV antibody
      • PLC 2 antibody
      • PLC gamma 2 antibody
      • PLC IV antibody
      • PLC-gamma-2 antibody
      • PLC-IV antibody
      • Plcg2 antibody
      • PLCG2_HUMAN antibody
      see all

    Anti-PLCG 2 (phospho Y1217 + Y1217) antibody [EP1404Y] 图像

    • All lanes : Anti-PLCG 2 (phospho Y1217 + Y1217) antibody [EP1404Y] (ab52610) at 1/50000 dilution

      Lane 1 : Raji cell lysate with no pervanadate
      Lane 2 : Raji cell lysate with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 150 kDa
    • ab52610 staining PLCG 2 (phospho Y1217) in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized; antigen retrieval was by heat mediation in Tris EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/50) for 30 minutes at 20°C. An undiluted HRP-conjugated mouse anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded human lymph node tissue using ab52610 at a dilution of 1/50.

    Anti-PLCG 2 (phospho Y1217 + Y1217) antibody [EP1404Y] (ab52610)参考文献

    ab52610 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9,0
    Sample Human Tissue sections (Stomach)
    Specification Stomach
    Permeabilization Yes - Wash Buffer from Dako with Tween
    Fixative Paraformaldehyde
    Username

    Mr. Rudolf Jung

    Verified customer

    提交于 Mar 30 2015

    Thank you for your enquiry.

    Below please find the protocol used to test this antibody in IHC-P. Please note that citrate buffer pH 6 was used as the retreival buffer.

    I hope this is helpful. Please contact me again if you have any f...

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    Thank you for your enquiry regarding ab52610.

    The phosphorylation of PLCG 2 at Tyrosine 1217 needs to be induced to be able to detect this specific modification. As the WB image on the datasheet indicates Raji cell lysate without stimulation ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"