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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PLCG 2 aa 1200 to the C-terminus (phospho Y1217).
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab52610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab52610 staining PLCG 2 (phospho Y1217) in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized; antigen retrieval was by heat mediation in Tris EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/50) for 30 minutes at 20°C. An undiluted HRP-conjugated mouse anti-rabbit IgG polyclonal was used as the secondary antibody.
ab52610 has not yet been referenced specifically in any publications.