Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
1/100 - 1/250.
Is unsuitable for Flow Cyt or IHC-P.
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Contains 1 C2 domain.
Contains 1 PH domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain. Contains 2 SH2 domains. Contains 1 SH3 domain.
Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
Information by UniProt
1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
5-bisphosphate phosphodiesterase gamma-2 antibody
Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] 图像
Western blot - PLCG 2 (phospho Y1197) antibody [EP1405Y] (ab75970)
All lanes :
Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] (ab75970) at 1/20000 dilution
Lane 1 :
Raji cell lysates un-treated
Lane 2 :
Raji cell lysates treated with pervanadate
Lysates/proteins at 10 µg per lane.
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size :
Observed band size :
150 kDa (
why is the actual band size different from the predicted?
Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] (ab75970)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"