存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
纯度 Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
1/100 - 1/250.
应用说明 Is unsuitable for Flow Cyt or IHC-P.
功能 The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
序列相似性 Contains 1 C2 domain. Contains 1 PH domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain. Contains 2 SH2 domains. Contains 1 SH3 domain.
翻译后修饰 Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
Information by UniProt
1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
5-bisphosphate phosphodiesterase gamma-2 antibody
Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] 图像
Western blot - PLCG 2 (phospho Y1197) antibody [EP1405Y] (ab75970)
All lanes : Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] (ab75970) at 1/20000 dilution Lane 1 : Raji cell lysates un-treated Lane 2 : Raji cell lysates treated with pervanadate Lysates/proteins at 10 µg per lane. Secondary HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size : 148 kDa Observed band size : 150 kDa ( why is the actual band size different from the predicted?)
Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] (ab75970)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"