RabMAb

Anti-PLCG 2 (phospho Y1197)抗体[EP1405Y] (ab75970)

概述

  • 产品名称Anti-PLCG 2 (phospho Y1197)抗体[EP1405Y]
    参阅全部 PLCG 2 一抗
  • 描述
    兔单克隆抗体[EP1405Y] to PLCG 2 (phospho Y1197)
  • 特异性ab75970 detects PLCG 2 phosphorylated on tyrosine 1197.
  • 经测试应用适用于: WB, IP, ICCmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding tyrosine 1197 of human PLCG 2.

  • 阳性对照
    • Raji cell lysates treated with pervanadate.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab75970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
IP 1/40.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    • 功能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
    • 序列相似性Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • 翻译后修饰Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase gamma-2 antibody
      • EC 3.1.4.11 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-2 antibody
      • Phospholipase C gamma 2 antibody
      • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
      • Phospholipase C-gamma-2 antibody
      • Phospholipase C-IV antibody
      • PLC 2 antibody
      • PLC gamma 2 antibody
      • PLC IV antibody
      • PLC-gamma-2 antibody
      • PLC-IV antibody
      • Plcg2 antibody
      • PLCG2_HUMAN antibody
      see all

    Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] 图像

    • All lanes : Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] (ab75970) at 1/20000 dilution

      Lane 1 : Raji cell lysates un-treated
      Lane 2 : Raji cell lysates treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 148 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] (ab75970)参考文献

    ab75970 has not yet been referenced specifically in any publications.

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