概述

  • 产品名称Anti-PLCG 2抗体
    参阅全部 PLCG 2 一抗
  • 描述
    小鼠多克隆抗体to PLCG 2
  • 经测试应用适用于: IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human PLCG 2 (AAH07565.1)

  • 阳性对照
    • Human liver tissue lysate, K-562 cell lysate, PLCG2 transfected 293T cell lysate and HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab89625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 23555801
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 148 kDa.

靶标

  • 功能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
  • 序列相似性Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • 翻译后修饰Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase gamma-2 antibody
    • EC 3.1.4.11 antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-2 antibody
    • Phospholipase C gamma 2 antibody
    • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
    • Phospholipase C-gamma-2 antibody
    • Phospholipase C-IV antibody
    • PLC 2 antibody
    • PLC gamma 2 antibody
    • PLC IV antibody
    • PLC-gamma-2 antibody
    • PLC-IV antibody
    • Plcg2 antibody
    • PLCG2_HUMAN antibody
    see all

Anti-PLCG 2 antibody 图像

  • Anti-PLCG 2 antibody (ab89625) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Predicted band size : 148 kDa
    Observed band size : 148 kDa
  • Anti-PLCG 2 antibody (ab89625) at 1 µg/ml + K-562 cell lysate at 50 µg

    Predicted band size : 148 kDa
    Observed band size : 148 kDa
  • All lanes : Anti-PLCG 2 antibody (ab89625) at 1 µg/ml

    Lane 1 : PLCG 2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 148 kDa

Anti-PLCG 2 antibody (ab89625)参考文献

This product has been referenced in:
  • Bernal-Quirós M  et al. BANK1 and BLK act through phospholipase C gamma 2 in B-cell signaling. PLoS One 8:e59842 (2013). IP ; Human . Read more (PubMed: 23555801) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa Cells)
Loading amount 20 µg
Specification HeLa Cells
Gel Running Conditions Non-reduced Denaturing (7.5% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 May 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"