概述

  • 产品名称Anti-Plastin L抗体[LPL4A.1]
    参阅全部 Plastin L 一抗
  • 描述
    小鼠单克隆抗体[LPL4A.1] to Plastin L
  • 特异性ab80729 recognizes Plastin L in a Ca2+ independent fashion but does not react with T- or I- Plastin. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: IHC-P, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human recombinant Plastin L protein

  • 表位Headpiece region of Plastin L
  • 阳性对照
    • Human polymorphonuclear neutrophils and monocytes, MCF-7, Jurkat cells.

性能

应用

Our Abpromise guarantee covers the use of ab80729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 70 kDa.
IP Use at 2 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration.

Fixed in methanol or 3% paraformaldehyde. Use at an assay dependent dilution.

靶标

  • 功能Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
  • 组织特异性Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
  • 序列相似性Contains 2 actin-binding domains.
    Contains 4 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
  • 翻译后修饰Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
    The N-terminus is blocked.
  • 细胞定位Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CP64 antibody
    • L plastin antibody
    • L-plastin antibody
    • Larval cuticle protein 1 antibody
    • LC64P antibody
    • LCP-1 antibody
    • LCP1 antibody
    • LPL antibody
    • Lplastin antibody
    • Lymphocyte cytosolic protein 1 antibody
    • Plastin 2 antibody
    • Plastin-2 antibody
    • PLS2 antibody
    • PLSL_HUMAN antibody
    see all

Anti-Plastin L antibody [LPL4A.1] 图像

  • Anti-Plastin L antibody [LPL4A.1] (ab80729) at 1 µg/ml + Human Cells-15 Lysate

    Predicted band size : 70 kDa
    Observed band size : 70 kDa

Anti-Plastin L antibody [LPL4A.1] (ab80729)参考文献

This product has been referenced in:
  • Lollike K  et al. Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. J Biol Chem 276:17762-9 (2001). Read more (PubMed: 11279160) »
  • Jones SL & Brown EJ FcgammaRII-mediated adhesion and phagocytosis induce L-plastin phosphorylation in human neutrophils. J Biol Chem 271:14623-30 (1996). Read more (PubMed: 8663066) »

See all 2 Publications for this product

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