Anti-Plastin L抗体(ab81454)

概述

  • 产品名称
    Anti-Plastin L抗体
    参阅全部 Plastin L 一抗
  • 描述
    兔多克隆抗体to Plastin L
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human Plastin L aa 504-553 (internal sequence). Synthetic peptide: LNILEEIGGG QKVNDDIIVN WVNETLREAK KSSSISSFKD PKISTSLPVL, corresponding to a region within internal sequence amino acids 504 - 553 of Human Plastin L (NP_002289)
    Database link: P13796

  • 阳性对照
    • Placenta lysate

性能

应用

Our Abpromise guarantee covers the use of ab81454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
  • 组织特异性
    Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
  • 序列相似性
    Contains 2 actin-binding domains.
    Contains 4 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
  • 翻译后修饰
    Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
    The N-terminus is blocked.
  • 细胞定位
    Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CP64 antibody
    • L plastin antibody
    • L-plastin antibody
    • Larval cuticle protein 1 antibody
    • LC64P antibody
    • LCP-1 antibody
    • LCP1 antibody
    • LPL antibody
    • Lplastin antibody
    • Lymphocyte cytosolic protein 1 antibody
    • Plastin 2 antibody
    • Plastin-2 antibody
    • PLS2 antibody
    • PLSL_HUMAN antibody
    see all

Anti-Plastin L antibody 图像

  • Anti-Plastin L antibody (ab81454) at 1 µg/ml (in 5% skim milk / PBS buffer) + placenta lysate at 10 µg/ml

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-Plastin L antibody (ab81454)参考文献

ab81454 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

提交于 Jan 31 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Spinal cord, Whole body)
Specification
Spinal cord, Whole body
Fixative
Paraformaldehyde
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
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Verified customer

提交于 May 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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