Anti-Plasminogen抗体[8E7] (ab38157)

概述

  • 产品名称Anti-Plasminogen抗体[8E7]
    参阅全部 Plasminogen 一抗
  • 描述
    小鼠单克隆抗体[8E7] to Plasminogen
  • 特异性Reacts with plasminogen, plasmin and angiostatin.
  • 经测试应用适用于: Other, ELISA, IHC-FoFr, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified human plasminogen.

性能

应用

Our Abpromise guarantee covers the use of ab38157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Other
AP
ELISA
IHC-FoFr
IHC-P
  • 应用说明AP: Use at an assay dependent dilution.
    ELISA: Use at an assay dependent dilution.
    IHC-F: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
      Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
    • 组织特异性Present in plasma and many other extracellular fluids. It is synthesized in the liver.
    • 疾病相关Defects in PLG are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.
      Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa.
    • 序列相似性Belongs to the peptidase S1 family. Plasminogen subfamily.
      Contains 5 kringle domains.
      Contains 1 PAN domain.
      Contains 1 peptidase S1 domain.
    • 结构域Kringle domains mediate interaction with CSPG4.
    • 翻译后修饰N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
      In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.
    • 细胞定位Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
    • Information by UniProt
    • 数据库链接
    • 形式Cleaved into the following 5 chains: 1.Plasmin heavy chain A2.Activation peptide3.Angiostatin4.Plasmin heavy chain A, short form5. Plasmin light chain B
    • 别名
      • Angiostatin antibody
      • DKFZp779M0222 antibody
      • Plasmin antibody
      • Plasmin heavy chain A antibody
      • Plasmin light chain B antibody
      • Plasminogen antibody
      • PLG antibody
      • PLMN_HUMAN antibody
      see all

    Anti-Plasminogen antibody [8E7] (ab38157)参考文献

    ab38157 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply.

    We do not carry a plasminogen ELISA kit that utilizes ab38157. However, we do know that ab38157 works well as a detection antibody with the clone 5H3 (ab10173) or 8F11 (ab10177) as the capture antibody.

    htt...

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    Thank you for your inquiry.

    The lab confirmed that ab38157 reacts with the nonoverlapping determinants on human plasminogen and plasmin molecules and it does not interact with the angiostatin portion (kringles 1-4) of human plasminogen.
    ...

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    Thank you for contacting us.

    We do have a Plasminogen Human ELISA kit as ab108893, but this has not been tested for cross-reactivity with angiostatin.

    http://www.abcam.com/Plasminogen-Human-ELISA-Kit-ab108893.html

    We do h...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"