Anti-Plasminogen抗体[3C2] (ab10178)


  • 产品名称Anti-Plasminogen抗体[3C2]
    参阅全部 Plasminogen 一抗
  • 描述
    小鼠单克隆抗体[3C2] to Plasminogen
  • 特异性Reacts with human plasminogen and plasmin molecules.
  • 经测试应用适用于: ELISA, IHC-P, Flow Cyt, Othermore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) (Human).



Our Abpromise guarantee covers the use of ab10178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Other Use at an assay dependent concentration.


  • 功能Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
    Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
  • 组织特异性Present in plasma and many other extracellular fluids. It is synthesized in the liver.
  • 疾病相关Defects in PLG are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.
    Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa.
  • 序列相似性Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 5 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • 结构域Kringle domains mediate interaction with CSPG4.
  • 翻译后修饰N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
    In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.
  • 细胞定位Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
  • Information by UniProt
  • 数据库链接
  • 形式Cleaved into the following 5 chains: 1.Plasmin heavy chain A2.Activation peptide3.Angiostatin4.Plasmin heavy chain A, short form5. Plasmin light chain B
  • 别名
    • Angiostatin antibody
    • DKFZp779M0222 antibody
    • Plasmin antibody
    • Plasmin heavy chain A antibody
    • Plasmin light chain B antibody
    • Plasminogen antibody
    • PLG antibody
    • PLMN_HUMAN antibody
    see all

Anti-Plasminogen antibody [3C2] 图像

  • ab10178 staining human brain tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in EDTA prior to blocking in 10% serum for 20 hours at 37°C.  The primary antibody was diluted 1/200 and incubated with the sample for 18 hours at 37°C.  A biotinylated horse anti-mouse IgG antibody, diluted 1/500, was used as the secondary.

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  • Overlay histogram showing HepG2 cells stained with ab10178 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10178, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Plasminogen antibody [3C2] (ab10178)参考文献

This product has been referenced in:
  • Barker R  et al. Plasminogen and plasmin in Alzheimer's disease. Brain Res 1355:7-15 (2010). IHC-P ; Human . Read more (PubMed: 20709033) »
  • Datta S  et al. Ultrasound-enhanced thrombolysis using Definity as a cavitation nucleation agent. Ultrasound Med Biol 34:1421-33 (2008). IHC-Fr ; Human . Read more (PubMed: 18378380) »

See all 2 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization No
Blocking step Serum as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C

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提交于 Jul 23 2008

Thank you very much for your email and patience. All the information that we have regarding these antibodies is located on the online datasheets. At this time, we don't have any information concerning the epitopes. For ELISA, ab10176 can be used as con...

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