Plasminogen活化剂抑制剂Type 1人Chromogenic Activity试剂盒(ab108894)

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概述

  • 产品名称
    Plasminogen活化剂抑制剂Type 1人Chromogenic Activity试剂盒
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Quantitative
  • 灵敏度
    0.5 IU/ml
  • 范围
    0.625 IU/ml - 20 IU/ml
  • 产品概述

    Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit (ab108894) has been developed to determine human type I plasminogen activator inhibitor (PAI-1) activity in cell culture supernatants. A fixed amount of tissue-type plasminogen activator (tPA) is added in excess to undiluted sample, which allows PAI-1 and tPA to form an inactive complex. The assay measures plasminogen activation by residual tPA in coupled assays that contain tPA, plasminogen, and a plsmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The absorbance of the pNA at 405 nm is inversely proportional to the PAI-1 enzymatic activity.

  • 说明

    Type I plasminogen activator inhibitor (PAI-1) is a 50 kDa serpin family member that inhibits tissue- and urokinase-type plaminogen activators (t-PA, u-PA). This protein appears to be an important regulator of plasminogen activation by t-PA and extracellular proteolysis by u-PA. The plasminogen activator proteolytic enzyme systems are important not only for fibrinolysis but also for extracellular matrix remodeling, and have been implicated in a number of normal and pathological processes including angiogensis, ovulation and embryogenesis, thrombotic and hemorrhagic disorders, connective tissue diseases, neoplasm and sepsis. PAI-1 is a prognosticator in breast cancer, gastric cancer, various forms of lung cancer and cervical cancer.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

应用

Our Abpromise guarantee covers the use of ab108894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

图片

  • Example of Standard Curve
    Example of Standard Curve

实验方案

文献

ab108894 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab108894？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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3 reviews or Q&A

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Hi, I was looking through the protocol for your Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit (ab108894) and while you add exogenous tPA to serum & plasma samples, this is NOT suggested for cell culture supernatants.  Is this a ...

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Thank you for getting in touch.


I can confirm that the tPA standard must be added to all the sample including cell culture supernatant for the Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Kit (ab108894).


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Will this kit react with mouse or rat samples?

Our lab has found that rat and mouse samples both show no significant activity with this particular kit. We would not advise using mouse or rat samples.

Buenas tardes
Estamos interesados en determinar la actividad de PAI-I en una serie de lineas celulares de carcinoma.
He visto que teneis disponible un ensayo chromogénico para la detección de PAI basado en su unión a tPA....

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Muchas gracias por contactarnos.
Todas las referencias, publicaciones, e información disponible sobre nuestros productos la compartimos en nuestra web para utilidad de los usuarios.
Lamentablemente para este kit no tenemos ninguna referencia ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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