概述

  • 产品名称Plasma膜Protein Extraction试剂盒
  • 检测类型Direct
  • 产品概述

    The Plasma Membrane Protein Extraction Kit provides optimized buffers and reagents for effective extraction of membrane proteins from mammalian tissues and cells. Unlike other available procedures that can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins), this kit was designed to not only extract the total cellular membrane proteins, but also purify the plasma membrane proteins specifically. The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.

  • 经测试应用适用于: Purificationmore details

性能

  • 相关性Membrane Protein Extraction is a method for extraction of membrane proteins from mammalian tissues and cells. Many procedures extract total cellular membrane proteins which is a combination of plasma and organelle membrane proteins.

应用

Our Abpromise guarantee covers the use of ab65400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Purification Use at an assay dependent concentration.

实验方案

Plasma Membrane Protein Extraction Kit (ab65400)参考文献

This product has been referenced in:
  • Subbarayal P  et al. EphrinA2 receptor (EphA2) is an invasion and intracellular signaling receptor for Chlamydia trachomatis. PLoS Pathog 11:e1004846 (2015). Human . Read more (PubMed: 25906164) »
  • Choveau FS  et al. The Role of the Carboxyl Terminus Helix C-D Linker in Regulating KCNQ3 K+ Current Amplitudes by Controlling Channel Trafficking. PLoS One 10:e0145367 (2015). Read more (PubMed: 26692086) »

See all 17 Publications for this product

Product Wall

Thank you for the update. I am glad to hear the replacement kit worked out better for you. Eliminating those extra steps will probably, as you found, give purer fractions but possibly at the cost of lower yield.

I am not sure why tubulin was g...

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Merci pour votre requête. J'ai contacté le laboratoire et ils m'ont confirmé que cette étape est pour équilibrer la "upper phase" solution. Il ne faux donc pas prendre la solution directement de la bouteille....

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This kit may be useful for extracting and retainingthe plasma membrane fraction without the plasma membrane proteins but it has not been specifically designed for this purpose. Please let us know how things go if you do choose to use ab65400 for this e...

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I can confirm that the kit uses an aqueous polymer two-phase system to separate plasma membranes. Most specifically it uses the PEG/ Dextran system and the plasma membrane tend to concentrate in the upper phase.


I am sorry we do not have ...

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1. The smaller cell number can decrease yield. Hence it is at the user's discretion to reduce cell numbers. For some cells, based on size and density of expression of membrane proteins, smaller cell number can still yield enough membrane proteins. If c...

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1. This kit isolates the plasma membrane associated proteins as well as transmembrane proteins on the plasma membrane. Organelle membrane proteins are not isolated. If total membrane proteins were desired, you would get all membrane proteins ...

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Yes, this will give you two sub-cellular fractions: plasma membrane and cytosolic. So you will be able to demonstrate the relative amounts of your protein of interest in each fraction using an assay such as western blotting, assuming you have an antibo...

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The proteins isolated when using this kit should be in their native conformation.

The frozen tissue cannot be pulverized with the Dounce homogenizer. This has to be done with a pulverizer or tissue grinder. Once ground, the buffer can be used to homogenize the bits in a Dounce homogenizer.

Fresh tissue can be homogenized di...

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You are obtaining a huge pellet after step 4. This is the low speed centrifugation at 700g and pellets down unbroken cells, nuclei and debris. Getting a huge pellet at this step is indicative of incomplete homogenization. The steps are correct but...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"