Recombinant fragment corresponding to amino acids 236-344 of Human PLAGL2
Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells); Human lymph node tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/250 - 1/500.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6.0
Shows weak transcriptional activatory activity.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers.
Information by UniProt
C2H2 type zinc finger protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-PLAGL2 antibody (ab121239)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121239 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-PLAGL2 antibody (ab121239)
All lanes : Anti-PLAGL2 antibody (ab121239) at 1/250 dilution Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2 : Negative control (vector only transfected HEK293T lysate) Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLAGL2 antibody (ab121239)
Immunohistochemical staining of Paraffin embedded Human lymph node tissue, using 1/35 ab121239, shows strong cytoplasmic positivity in reaction center cells.
This product has been referenced in:
et al. Pleomorphic adenoma gene like-2 induces epithelial-mesenchymal transition via Wnt/ß-catenin signaling pathway in human colorectal adenocarcinoma. Oncol Rep 37:1961-1970 (2017).
Read more (PubMed: 28259923) »
et al. Overexpression of Pleomorphic Adenoma Gene-Like 2 Is a Novel Poor Prognostic Marker of Prostate Cancer. PLoS One 11:e0158667 (2016).
Read more (PubMed: 27537362) »
See all 3 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"