Anti-PLAGL1 / ZAC抗体(ab90472)

概述

  • 产品名称Anti-PLAGL1 / ZAC抗体
    参阅全部 PLAGL1 / ZAC 一抗
  • 描述
    兔多克隆抗体to PLAGL1 / ZAC
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 72-121 (NRKDHLKNHL QTHDPNKMAF GCEECGKKYN TMLGYKRHLA LHAASSGDLT) of Human PLAGL1/ ZAC (NP_006709)

  • 阳性对照
    • Human fetal kidney lysate

性能

应用

Our Abpromise guarantee covers the use of ab90472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Shows weak transcriptional activatory activity. Transcriptional regulator of the type 1 receptor for pituitary adenylate cyclase-activating polypeptide.
  • 序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 7 C2H2-type zinc fingers.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp781P1017 antibody
    • Lost on transformation 1 antibody
    • Lost on Transformation antibody
    • LOT-1 antibody
    • LOT1 antibody
    • MGC126275 antibody
    • MGC126276 antibody
    • PLAG like 1 antibody
    • PLAGL1 antibody
    • PLAL1_HUMAN antibody
    • Pleiomorphic adenoma gene like 1 antibody
    • Pleiomorphic adenoma gene like protein 1 antibody
    • Pleiomorphic adenoma-like protein 1 antibody
    • Tumor supressor ZAC antibody
    • ZAC antibody
    • ZAC tumor supressor antibody
    • ZAC1 antibody
    • Zinc finger protein PLAGL1 antibody
    see all

Anti-PLAGL1 / ZAC antibody 图像

  • Anti-PLAGL1 / ZAC antibody (ab90472) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal kidney lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : <22 kDa. We are unsure as to the identity of these extra bands.

Anti-PLAGL1 / ZAC antibody (ab90472)参考文献

ab90472 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application ChIP
Sample Cow Tissue lysate - whole (Day ~105 fetal heart and kidney)
Negative control PCR negative control: non-template control ChIP negative control: protein A/G magnetic beads ChIP negative control regions: IGF2 intron1 and GAPDH exon1* *Note: PLAGL1 has not been reported to bind to these two regions in mice and human. Particularly, it has been shown that PLAGL1 does not bind to IGF2 in mouse (Varrault et al., 2006 Dev Cell). In addition, the PLAGL1 binding motif in mouse GLUT4 (and putative motif in cow) was not found in IGF2 intron1 and GAPDH exon1 in cow.
Specification Day ~105 fetal heart and kidney
Detection step Other
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde (Sigma F8775)
Positive control PCR positive control: genomic DNA (bovine fetal heart) Chromatin input: 1% of the chromatin used for immunoprecipitation ChIP positive control region: GLUT4 promoter region* *Note: PLAGL1 has been reported to bind to GLUT4 promoter region in mice (Czubryt MP et al., 2010 Journal of Biological Chemistry). The PLAGL1 binding motif in mouse GLUT4 is conserved in cow.
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提交于 Apr 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"