- 数据表
- 具体的参考文献 (3)
- 实验方案
概述
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产品名称Anti-Placental lactogen抗体
参阅全部 Placental lactogen 一抗 -
描述兔多克隆抗体to Placental lactogen
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宿主Rabbit
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经测试应用适用于: ICC/IF, WB, IHC-Pmore details
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种属反应性与反应: Human
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免疫原
Purified human placental lactogen
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阳性对照
- Placenta
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, BSA -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab15554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. | |
| IHC-P | 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能Produced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. Does not interact with GHR but only activates PRLR through zinc-induced dimerization.
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序列相似性Belongs to the somatotropin/prolactin family.
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细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 1442 Human
- Entrez Gene: 1443 Human
- Omim: 118820 Human
- Omim: 150200 Human
- SwissProt: P01243 Human
- Unigene: 654390 Human
- Unigene: 654515 Human
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别名
- Choriomammotropin antibody
- Chorionic somatomammotropin A antibody
- chorionic somatomammotropin B antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental lactogen antibody (ab15554)ab15554 staining Human placenta by Immunohistochemistry (FFPE-sections).
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Western blot - Anti-Placental lactogen antibody (ab15554)This image is courtesy of an Abreview submitted by Dr Stoil DmitrovAnti-Placental lactogen antibody (ab15554) at 1/100 dilution + Human recombinant hPL at 0.1 µg
Secondary
HRP conjugated donkey anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Additional bands at: 10 kDa, 14 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-Placental lactogen antibody (ab15554)This image is courtesy of an Abreview submitted by Dr Stoil Dmitrovab15554 at 1/200 staining human BeWo (placenta choriocarcinoma) cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A rhodamine conjugated goat anti-rabbit antibody was used as the secondary. The left hand panel of the image shows DAPI counterstain. -
Western blot - Anti-Placental lactogen antibody (ab15554)Anti-Placental lactogen antibody (ab15554) at 1/25 dilution + Human placenta lysate
Predicted band size: 25 kDa
Observed band size: 23 kDa (why is the actual band size different from the predicted?)
实验方案
文献
This product has been referenced in:
- Drukker M et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). ICC/IF ; Human . Read more (PubMed: 22634564) »
- Adachi K et al. Role of SOX2 in maintaining pluripotency of human embryonic stem cells. Genes Cells : (2010). Read more (PubMed: 20384793) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"