RabMAb

Anti-Placental alkaline phosphatase (PLAP)抗体[SP15] (ab16695)

概述

  • 产品名称Anti-Placental alkaline phosphatase (PLAP)抗体[SP15]
    参阅全部 Placental alkaline phosphatase (PLAP) 一抗
  • 描述
    兔单克隆抗体[SP15] to Placental alkaline phosphatase (PLAP)
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Recombinant full length protein (Human).

  • 表位Not determined
  • 阳性对照
    • Seminoma and placenta
  • 常规说明


    This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: Tissue culture supernatant
  • 纯度Tissue culture supernatant
  • Primary antibody说明This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
  • 克隆单克隆
  • 克隆编号SP15
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab16695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

WB Use at an assay dependent concentration. PubMed: 16424182

靶标

  • 序列相似性Belongs to the alkaline phosphatase family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alkaline phosphatase antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase Regan isozyme antibody
    • ALP antibody
    • Alp1 antibody
    • ALPP antibody
    • FLJ61142 antibody
    • Germ-cell alkaline phosphatase antibody
    • nagao isozyme antibody
    • OTTHUMP00000164354 antibody
    • PALP antibody
    • Placental alkaline phosphatase 1 antibody
    • placental heat-stable alkaline phosphatase antibody
    • placental type antibody
    • PLAP antibody
    • PLAP-1 antibody
    • PLAP1 antibody
    • PPB1_HUMAN antibody
    see all

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] 图像

  • Human seminoma stained with ab16695.
  • Immunohistochemical analysis of paraffin-embedded Human seminoma tissue labelling Alkaline Phosphatase with ab137879 at 1/50 dilution.

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)参考文献

This product has been referenced in:
  • Saffarini CM  et al. Induction and persistence of abnormal testicular germ cells following gestational exposure to di-(n-butyl) phthalate in p53-null mice. J Androl 33:505-13 (2012). IHC-P ; Mouse . Read more (PubMed: 21868749) »
  • Schmid F  et al. Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Degradation by Alkaline Phosphatase. J Biol Chem 287:32525-34 (2012). WB . Read more (PubMed: 22851169) »

See all 4 Publications for this product

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Here are the answers to your questions:

1. ab16517 - immunogen is Full length native protein (purified) (Human) and epitope has not been mapped
2. ab16695 - immunogen is Full length native protein (p...

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Please see the western blot in following reference noted under the 'References' section of the online datasheet for ab16695: Taylor DD et al. Pregnancy-associated exosomes and their modulation of T cell signaling. J Immunol 176:1534-42 (2006). WB; ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"