重组Anti-Placental alkaline phosphatase (PLAP)抗体[EPR6141] (ab133602)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6141] to Placental alkaline phosphatase (PLAP)
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Placental alkaline phosphatase (PLAP)抗体[EPR6141]
参阅全部 Placental alkaline phosphatase (PLAP) 一抗 -
描述
兔单克隆抗体[EPR6141] to Placental alkaline phosphatase (PLAP) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Placental alkaline phosphatase (PLAP) aa 150-250 (extracellular). The exact sequence is proprietary.
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阳性对照
- WB: Human placenta, and seminoma tissue lysates, and HeLa cell lysate. IHC-P: Human dysgerminoma and placenta tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 5.10 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6141 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133602于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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序列相似性
Belongs to the alkaline phosphatase family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 250 Human
- Omim: 171800 Human
- SwissProt: P05187 Human
- Unigene: 284255 Human
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别名
- Alkaline phosphatase antibody
- Alkaline phosphatase placental antibody
- Alkaline phosphatase placental type antibody
see all
图片
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All lanes : Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ALPP knockout HeLa cell lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : Human heart tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab133602 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab133602 Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] was shown to specifically react with Placental alkaline phosphatase (PLAP) in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261763 (knockout cell lysate ab256836) was used. Wild-type and Placental alkaline phosphatase (PLAP) knockout samples were subjected to SDS-PAGE. ab133602 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602) at 1/10000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Human seminoma tissue lysate
Lane 3 : HeLa cell lysate at 10 µg
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
ab133602 showing positive staining in Normal placenta tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human dysgerminoma tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab133602 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab133602 showing negative staining in Normal liver tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab133602 showing positive staining in Seminoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab133602 showing negative staining in Normal colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab133602 showing negative staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab133602 被引用在 17 文献中.
- Chen Z et al. Pathogenic mechanisms of preeclampsia with severe features implied by the plasma exosomal mirna profile. Bioengineered 12:9140-9149 (2021). PubMed: 34696680
- Herrera-Van Oostdam AS et al. Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation. Int J Mol Med 46:546-560 (2020). PubMed: 32626972
- Kohno T et al. Reelin-Nrp1 Interaction Regulates Neocortical Dendrite Development in a Context-Specific Manner. J Neurosci 40:8248-8261 (2020). PubMed: 33009002
- Bing T et al. Aptameric Probe Specifically Binding Protein Heterodimer Rather Than Monomers. Adv Sci (Weinh) 6:1900143 (2019). PubMed: 31179220
- Xu E et al. Asymmetric expression of GPR126 in the convex/concave side of the spine is associated with spinal skeletal malformation in adolescent idiopathic scoliosis population. Eur Spine J 28:1977-1986 (2019). PubMed: 31079250