Anti-Placental alkaline phosphatase (PLAP)抗体[8B6] (ab33)

概述

  • 产品名称Anti-Placental alkaline phosphatase (PLAP)抗体[8B6]
    参阅全部 Placental alkaline phosphatase (PLAP) 一抗
  • 描述
    小鼠单克隆抗体[8B6] to Placental alkaline phosphatase (PLAP)
  • 特异性Human placental alkaline phosphatase, Regan and Nagao isoenzymes. No cross reaction with other isoenzymes of AP.
  • 经测试应用适用于: ELISA, IHC-P, RIA, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    HEp-2 cells with boosted surface expression of PLAP

  • 阳性对照
    • Placenta.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆单克隆
  • 克隆编号8B6
  • 骨髓瘤P3-NS1/1-Ag4-1
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab33 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-P 1/250 - 1/500.
RIA Use at an assay dependent concentration.
IHC-Fr 1/250 - 1/500.

靶标

  • 序列相似性Belongs to the alkaline phosphatase family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alkaline phosphatase antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase Regan isozyme antibody
    • ALP antibody
    • Alp1 antibody
    • ALPP antibody
    • FLJ61142 antibody
    • Germ-cell alkaline phosphatase antibody
    • nagao isozyme antibody
    • OTTHUMP00000164354 antibody
    • PALP antibody
    • Placental alkaline phosphatase 1 antibody
    • placental heat-stable alkaline phosphatase antibody
    • placental type antibody
    • PLAP antibody
    • PLAP-1 antibody
    • PLAP1 antibody
    • PPB1_HUMAN antibody
    see all

Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33)参考文献

This product has been referenced in:
  • Sharp TM  et al. Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal. PLoS One 5:e13130 (2010). ICC/IF ; Human . Read more (PubMed: 20976190) »
  • Zhang L  et al. Alteration in the IL-2 signal peptide affects secretion of proteins in vitro and in vivo. J Gene Med 7:354-65 (2005). ELISA ; Human . Read more (PubMed: 15619290) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry. Unfortunately we have not tested ab33 against mouse so we do not know whether it is suitable. The antibody was raised against the whole protein, and although the mouse and human protein show 75% homology we do not know wh...

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Thank you for your enquiry. According to the database SwissProt the MW of the precursor PLAP is 57kDa and it is glycosylated therefore its weight should be higher than this. A paper by Webb et al (Placenta. 1986 Sep-Oct;7(5):405-15.Isolation of pl...

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I'm afraid that we do not have any data regarding the suitablity of this antibody for WB.

This antibody has not been tested in flow cytometry.

This antibody has not been tested in immunofluorescence.

This monoclonal was raised against the whole protein, and in the absence of any epitope mapping studies, I am afraid that we do not know against which part of the protein the antibody is reactive.

Vlad We do not have any data on this antibody's inhibiting activity in vivo or in vitro. We are about to implement a trial 'try risk free' scheme which says that if an antibody is purchased and used in an experiment outside that written on the datashe...

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This antibody has not yet been tested in Western blotting.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"