Anti-PLA2R抗体(ab99466)
Key features and details
- Goat polyclonal to PLA2R
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PLA2R抗体
参阅全部 PLA2R 一抗 -
描述
山羊多克隆抗体to PLA2R -
宿主
Goat -
特异性
ab99466 is expected to recognize both isoforms (NP_031392.3; NP_001007268.1). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Cow, Orangutan -
免疫原
Synthetic peptide:
C-EKKKDTPKQHGT
, corresponding to internal sequence amino acids 946-957 of Human PLA2R (NP_031392.3; NP_001007268.1). -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab99466 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99466于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 153 kDa.
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 153 kDa. 1 hour primary incubation is recommended for this product. |
靶标
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功能
Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B. -
组织特异性
Present in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level. -
序列相似性
Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
结构域
C-type lectin domains 3-5 mediate the interaction with phospholipase PLA2G1B.
The endocytosis signal probably mediates endocytosis via clathrin-coated pits. -
翻译后修饰
The secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 317777 Cow
- Entrez Gene: 22925 Human
- Entrez Gene: 100173143 Orangutan
- Entrez Gene: 100009180 Rabbit
- Omim: 604939 Human
- SwissProt: P49259 Cow
- SwissProt: Q13018 Human
- SwissProt: Q5R880 Orangutan
see all -
别名
- 180 kDa secretory phospholipase A2 receptor antibody
- C-type lectin domain family 13 member C antibody
- CLEC13C antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab99466 尚未被引用在任何文献中。