Anti-PLA2R抗体[12-6-5] (ab211490)
Key features and details
- Mouse monoclonal [12-6-5] to PLA2R
- Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG2b
概述
-
产品名称
Anti-PLA2R抗体[12-6-5]
参阅全部 PLA2R 一抗 -
描述
小鼠单克隆抗体[12-6-5] to PLA2R -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human PLA2R aa 1-700. corresponding to extracellular sequence NC3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CTLD3) expressed in HEK 293-EBNA-1 cells
Database link: Q13018 -
阳性对照
- WB: Recombinant extracellular domain (NC8) and N terminal fragment (NC3; immunogen) of human PLA2R. ICC/IF: PLA2R over-expressing podocytes. IHC-P: Normal human kidney and membranous nephropathy kidney tissues. Flow Cytometry: PLA2R over-expressing podocytes.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解离常数(KD)
KD = 5.40 x 10 -10 M Learn more about KD -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS
Sterilization by membrane filtration. -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
12-6-5 -
骨髓瘤
Sp2 -
同种型
IgG2b -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab211490于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/5000 - 1/10000. Predicted molecular weight: 169 kDa.
|
|
Flow Cyt |
1/200.
|
|
ICC/IF |
1/400.
|
|
IHC-P |
1/5000 - 1/20000.
Perform heat mediated antigen retrieval at pH 8 before commencing with IHC staining protocol. See attached document for protocol details. |
说明 |
---|
WB
1/5000 - 1/10000. Predicted molecular weight: 169 kDa. |
Flow Cyt
1/200. |
ICC/IF
1/400. |
IHC-P
1/5000 - 1/20000. Perform heat mediated antigen retrieval at pH 8 before commencing with IHC staining protocol. See attached document for protocol details. |
靶标
-
功能
Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B. -
组织特异性
Present in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level. -
序列相似性
Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
结构域
C-type lectin domains 3-5 mediate the interaction with phospholipase PLA2G1B.
The endocytosis signal probably mediates endocytosis via clathrin-coated pits. -
翻译后修饰
The secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 22925 Human
- Omim: 604939 Human
- SwissProt: Q13018 Human
- Unigene: 410477 Human
-
别名
- 180 kDa secretory phospholipase A2 receptor antibody
- C-type lectin domain family 13 member C antibody
- CLEC13C antibody
see all
图片
-
All lanes : Anti-PLA2R antibody [12-6-5] (ab211490) at 1/10000 dilution
Lane 1 : Recombinant extracellular domain of human PLA2R (NC8)
Lane 2 : Recombinant N-terminus fragment of human PLA2R (NC3)
Lysates/proteins at 10 µg per lane.
Predicted band size: 169 kDa -
Immunohistochemical analysis of membranous nephropathy kidney tissue labeling PLA2R at 1/5000 dilution followed by DAB detection.
-
Immunofluorescent analysis of PLA2R over-expressing podocytes labeling PLA2R at 1/400 dilution (green).
-
Flow cytometry analysis of wild type (dark blue line) and over-expressing PLA2R podocytes (low expressor, green line; high expressor, red line) labeling PLA2R using ab211490 at 1/200 dilution. Negative controls were unlabelled cells (turquoise line) and mouse IgG (grey line).
-
Immunohistochemical analysis of normal human kidney tissue labeling PLA2R at 1/5000 dilution followed by DAB detection.
-
Immunohistochemical analysis of normal human kidney tissue labeling PLA2R at 1/5000 dilution followed by DAB detection.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab211490 被引用在 3 文献中.
- Luo J et al. Seropositive PLA2R-associated membranous nephropathy but biopsy-negative PLA2R staining. Nephrol Dial Transplant N/A:N/A (2020). PubMed: 33236093
- Watanabe K et al. Human soluble phospholipase A2 receptor is an inhibitor of the integrin-mediated cell migratory response to collagen-I. Am J Physiol Cell Physiol 315:C398-C408 (2018). PubMed: 29791208
- Fresquet M et al. PLA2R binds to the annexin A2-S100A10 complex in human podocytes. Sci Rep 7:6876 (2017). WB, ICC/IF ; Human . PubMed: 28761153