Recombinant protein fragment containing a sequence corresponding to a region within amino acids 379 and 602 (Q68DD2) of PLA2G4F
293T, A431 whole cell lysate, Raji whole cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78.99% PBS, 1% BSA, 20% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with 10 mM citrate buffer pH 6 or tris-EDTA buffer pH 8 before commencing with IHC staining protocol.
1/500 - 1/3000. Predicted molecular weight: 71 kDa.
Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine.
Contains 1 C2 domain.
Contains 1 PLA2c domain.
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).
Cytoplasm > cytosol. Lysosome membrane. Translocates to lysosomal membranes in a calcium-dependent fashion.
Information by UniProt
Cytosolic phospholipase A2 zeta antibody
Western blot - Anti-PLA2G4F antibody (ab111551)
Anti-PLA2G4F antibody (ab111551) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 71 kDa 7.5% SDS page
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2G4F antibody (ab111551)
ab111551 at 1/500 dilution, staining PLA2G4F by IHC-P (gastric ca).
has not yet been referenced specifically in any publications.
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