Anti-PLA2G3抗体(ab62197)

概述

  • 产品名称Anti-PLA2G3抗体
    参阅全部 PLA2G3 一抗
  • 描述
    兔多克隆抗体to PLA2G3
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human PLA2G3 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • Human colonic carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab62197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2.5 µg/ml.

靶标

  • 功能PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Shows an 11-fold preference for phosphatidylglycerol over phosphatidylcholine (PC). Preferential cleavage: 1-palmitoyl-2-linoleoyl-phosphatidylethanolamine (PE) > 1-palmitoyl-2-linoleoyl-PC > 1-palmitoyl-2-arachidonoyl-PC > 1-palmitoyl-2-arachidonoyl-PE. Plays a role in ciliogenesis.
  • 组织特异性Expressed in kidney, heart, liver, and skeletal muscle. Also present in placenta and peripheral blood leukocytes. Not detected in brain, colon, thymus, spleen and small intestine. In lung, expressed in bronchial epithelial cells and alveolar macrophages, but scarcely detected in alveolar epithelium, arterial walls and interstitial fibroblasts (at protein level). In joints of osteoarthritis and rheumatoid arthritis, expressed in endothelial cells (at protein level). In normal heart, detected in some vessels. In myocardial tissues with acute infarction, expressed in vascular endothelial cells adjacent to cardiomyocytes and those in lesions with granulation. Expression in cardiomyocytes is scarce (at protein level). In uterus, breast and colon cancers, detected in tumor cells and neighboring microvascular endothelium, but not in normal glandular tissues (at protein level).
  • 序列相似性Belongs to the phospholipase A2 family.
  • 翻译后修饰N-glycosylation does not affect the catalytic activity, but is required for proper secretion. A nonglycosylated form was observed in several cell types.
    In several cell types, the N- and C-termini are cleaved off.
  • 细胞定位Secreted. Cell membrane. Cytoplasm > cytoskeleton > centrosome > centriole.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 3.1.1.4 antibody
    • GIII sPLA2 antibody
    • Group 3 secretory phospholipase A2 antibody
    • Group III secretory phospholipase A2 antibody
    • PA2G3_HUMAN antibody
    • Phosphatidylcholine 2 acylhydrolase GIII antibody
    • Phosphatidylcholine 2-acylhydrolase 3 antibody
    • phospholipase A2 group III antibody
    • PLA2G3 antibody
    • sPLA2-III antibody
    • SPLA2III antibody
    see all

Anti-PLA2G3 antibody 图像

  • Immunohistochemical analysis of paraffin embedded colonic carcinoma tissue, using ab62197 at 2.5µg/ml.

Anti-PLA2G3 antibody (ab62197)参考文献

ab62197 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your response.
Currently, we can't offer customer made peptide synthesis. It is very likely that we currently we do not have specific antibodies which could target the central S domain of the PLA2g3 protein (149-291 of the human PLA2...

Read More

Thank you for your enquiry.
These two antibodies are from two different sources, the manufacturing and the characterization processes are different thus the tested applications are not the same as you can see of the product datasheets.
I can ...

Read More

Thank you for your inquiry.
The exact sequence used to generate this antibody is considered proprietary but I can release that it is taken from within the general range of aa 160-210 of the human protein according to NP_056530.2, so it is within a...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"