概述

  • 产品名称Anti-PLA2G12B抗体
    参阅全部 PLA2G12B 一抗
  • 描述
    兔多克隆抗体to PLA2G12B
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human PLA2G12B (N terminal). A 15 amino acid peptide from near the N terminal residues of human PLA2G12B.
    Database link: NP_115951

  • 阳性对照
    • WB: Mouse liver lysate. IHC-P: Human fetal liver tissue. ICC/IF: MCF7 cells.

性能

  • 形式Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/200 - 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/100 - 1/500.

靶标

  • 相关性Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]).
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • 2010002E04Rik antibody
    • FKSG71 antibody
    • Group XIIB phospholipase A2 antibody
    • Group XIII secreted phospholipase A2 antibody
    • GXIIB antibody
    • GXIIIsPLA2 antibody
    • MGC138151 antibody
    • Phospholipase A2, group XIIB antibody
    • PLA2G12B antibody
    • PLA2G13 antibody
    see all

Anti-PLA2G12B antibody 图像

  • Anti-PLA2G12B antibody (ab85069) at 1/250 dilution + mouse liver lysates

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
  • ICC/IF image of ab85069 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85069, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFa-fixed paraffin-embedded sections) analysis of human fetal liver tissue labelling PLA2G12B with ab85069 at a dilution of 1/100.

Anti-PLA2G12B antibody (ab85069)参考文献

This product has been referenced in:
  • Li X  et al. Hepatocyte nuclear factor 4a and downstream secreted phospholipase A2 GXIIB regulate production of infectious hepatitis C virus. J Virol 88:612-27 (2014). Read more (PubMed: 24173221) »

See 1 Publication for this product

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Thank you for contacting us. The liver lysate was not prepared in any special way. It was a standard lysis of fresh mouse liver in RIPA buffer with protease inhibitors. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"