RabMAb

Anti-PKR (phospho T451)抗体[EPR2152Y] (ab81303)

概述

  • 产品名称Anti-PKR (phospho T451)抗体[EPR2152Y]
    参阅全部 PKR 一抗
  • 描述
    兔单克隆抗体[EPR2152Y] to PKR (phospho T451)
  • 特异性ab81303 only detects PKR phosphorylated on Threonine 451.
  • 经测试应用适用于: WBmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Thr451 of Human PKR.

  • 阳性对照
    • WB: HeLa cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号EPR2152Y
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
    • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
      Contains 2 DRBM (double-stranded RNA-binding) domains.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Double stranded RNA activated protein kinase; antibody
      • E2AK2_HUMAN antibody
      • eIF-2A protein kinase 2 antibody
      • EIF2AK1 antibody
      • EIF2AK2 antibody
      • Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
      • Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
      • HGNC:9437 antibody
      • Interferon induced double stranded RNA activated protein kinase antibody
      • Interferon inducible elF2 alpha kinase antibody
      • Interferon inducible RNA dependent protein kinase antibody
      • Interferon-induced, double-stranded RNA-activated protein kinase antibody
      • Interferon-inducible RNA-dependent protein kinase antibody
      • MGC126524 antibody
      • P1/eIF-2A protein kinase antibody
      • P1/eIF2A protein kinase antibody
      • p68 kinase antibody
      • PKR antibody
      • PPP1R83 antibody
      • PRKR antibody
      • Protein kinase interferon inducible double stranded RNA dependent antibody
      • Protein kinase RNA activated antibody
      • Protein kinase RNA-activated antibody
      • Protein phosphatase 1 regulatory subunit 83 antibody
      • Serine/threonine protein kinase TIK antibody
      • Tyrosine protein kinase EIF2AK2 antibody
      see all

    Anti-PKR (phospho T451) antibody [EPR2152Y] 图像

    • All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303) at 1/2000 dilution

      Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma) treated with Interferon-a and Calyculin A whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 62 kDa

      Purified format.

      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303) at 1/2000 dilution (undiluted)

      Lane 1 : HeLa cell lysate un-treated
      Lane 2 : HeLa cell lysate treated with IFN-alpha

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)参考文献

    This product has been referenced in:
    • Khaperskyy DA  et al. Influenza A virus inhibits cytoplasmic stress granule formation. FASEB J 26:1629-39 (2012). Read more (PubMed: 22202676) »
    • Siddiqui MA & Malathi K RNase L induces autophagy via c-Jun N-terminal kinase and double-stranded RNA-dependent protein kinase signaling pathways. J Biol Chem 287:43651-64 (2012). WB . Read more (PubMed: 23109342) »

    See all 4 Publications for this product

    Product Wall

    Thank you for contacting us.

    The antibody is specific to phosphorylation site of T451, the datasheet has been updated.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or info...

    Read More

    Der Antikörper sollte dies eigentlich unbeschadet überstanden haben. Wir versenden unsere Antikörper so z.B. auch nach Australien oder Thailand, und da kann es schon einmal zu Transitzeiten von einer Woche kommen, und bisher haben wir noch keine erhöht...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Lung epithelial)
    Loading amount 10 µg
    Specification Lung epithelial
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

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    Verified customer

    提交于 Jul 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"