Anti-PKR (phospho T446)抗体[E120] - ChIP Grade (ab32036)


  • 产品名称Anti-PKR (phospho T446)抗体[E120] - ChIP Grade
    参阅全部 PKR 一抗
  • 描述
    兔单克隆抗体[E120] to PKR (phospho T446) - ChIP Grade
  • 特异性The antibody only detects PKR phosphorylated on Threonine 446.
  • 经测试应用适用于: ChIP, IHC-P, WB, IPmore details
    不适用于: Flow Cyt or ICC/IF
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Thr446 of human PKR (Interferon-inducible RNA-dependent protein kinase)

  • 阳性对照
    • Calynculin A and TNF alpha treated HeLa cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:
    Anti-PKR (phospho T446) antibody (HRP) [E120] (ab195850)



Our Abpromise guarantee covers the use of ab32036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use a concentration of 4 µg/ml.
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).Can be blocked with PKR (phospho T446) peptide (ab181660).
IP 1/10.
  • 应用说明Is unsuitable for Flow Cyt or ICC/IF.
  • 靶标

    • 功能Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
    • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
      Contains 2 DRBM (double-stranded RNA-binding) domains.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Double stranded RNA activated protein kinase; antibody
      • E2AK2_HUMAN antibody
      • eIF-2A protein kinase 2 antibody
      • EIF2AK1 antibody
      • EIF2AK2 antibody
      • Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
      • Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
      • HGNC:9437 antibody
      • Interferon induced double stranded RNA activated protein kinase antibody
      • Interferon inducible elF2 alpha kinase antibody
      • Interferon inducible RNA dependent protein kinase antibody
      • Interferon-induced, double-stranded RNA-activated protein kinase antibody
      • Interferon-inducible RNA-dependent protein kinase antibody
      • MGC126524 antibody
      • P1/eIF-2A protein kinase antibody
      • P1/eIF2A protein kinase antibody
      • p68 kinase antibody
      • PKR antibody
      • PPP1R83 antibody
      • PRKR antibody
      • Protein kinase interferon inducible double stranded RNA dependent antibody
      • Protein kinase RNA activated antibody
      • Protein kinase RNA-activated antibody
      • Protein phosphatase 1 regulatory subunit 83 antibody
      • Serine/threonine protein kinase TIK antibody
      • Tyrosine protein kinase EIF2AK2 antibody
      see all

    Anti-PKR (phospho T446) antibody [E120] - ChIP Grade 图像

    • All lanes : Anti-PKR (phospho T446) antibody [E120] - ChIP Grade (ab32036) at 1/1000 dilution (purified)

      Lane 1 : HeLa (human cervix adenocarcinoma) treated with Calyculin A and TNF-alpha whole cell lysates
      Lane 2 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-PKR (phospho T446) antibody [E120] - ChIP Grade (ab32036) at 1/1000 dilution (unpurified)

      Lane 1 : Hela cell lysate
      Lane 2 : Hela cell lysate + IFN + CalyculinA
      Lane 3 : Hela cell lysate + Pptase

      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of PKR (phospho T446) expression in normal colon tissue, using 1/50 unpurified ab32036.

    Anti-PKR (phospho T446) antibody [E120] - ChIP Grade (ab32036)参考文献

    This product has been referenced in:
    • Pfaller CK  et al. Measles virus C protein impairs production of defective copyback double-stranded viral RNA and activation of protein kinase R. J Virol 88:456-68 (2014). WB . Read more (PubMed: 24155404) »
    • Nejepinska J  et al. Reporters transiently transfected into mammalian cells are highly sensitive to translational repression induced by dsRNA expression. PLoS One 9:e87517 (2014). WB ; Human . Read more (PubMed: 24475301) »

    See all 24 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (CD4+ T cell)
    Gel Running Conditions Reduced Denaturing (10% acrylamide)
    Loading amount 20000 cells
    Treatment before or after pIC addition (10 minutes 30°C)
    Specification CD4+ T cell
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    提交于 Nov 20 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (293T)
    Loading amount 20000 cells
    Specification 293T
    Treatment 100ng poly(I:C) for 6 hous
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

    Abcam user community

    Verified customer

    提交于 May 09 2013

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    I have read the details you have kindly provided and have following further questions

    - What is the problem with these products...

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    Vielen Dank für Ihre Anfrage.

    Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an, da wir bei über 90.000Produkten gar nicht die logistischen Möglichkeiten haben, neben unserer eigentlichen Produktpallette...

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    Thank you for contacting us.

    I am sorry that the free of charge replacement has also regrettably not worked for you on this occasion. We appreciate you contacting us and this information will remain on our quality monitoring records.

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    Thank you for your note.

    This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab32036. For your information, the internal reference note for this cred...

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    Thank you very much for your updates.

    I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in th...

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    Thank you for your update.

    Could you please specific and explain how this particular phosphorylation at T446 position has been induced? Has the customer used any inducer or any positive control?

    I look forward to hearing from you soon.

    Thank you for your enquiry regarding ab32036 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Additionally, thank you for supplying an image, as this has...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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