重组Anti-PKR (phospho T446)抗体[E120] (ab32036)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E120] to PKR (phospho T446)
- Suitable for: WB, IP
- Reacts with: Human, Pig
Related conjugates and formulations
概述
-
产品名称
Anti-PKR (phospho T446)抗体[E120]
参阅全部 PKR 一抗 -
描述
兔单克隆抗体[E120] to PKR (phospho T446) -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human, Pig -
免疫原
-
阳性对照
- WB: HeLa treated with Calyculin A and TNF-alpha whole cell lysate; Calynculin A and IFN treated HeLa cell lysate. IHC-P: Human colon tissue. IP: HeLa
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E120 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32036于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (6) |
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
|
IP |
1/10.
|
说明 |
---|
WB
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa). |
IP
1/10. |
靶标
-
功能
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. - Information by UniProt
-
数据库链接
- Entrez Gene: 5610 Human
- Omim: 176871 Human
- SwissProt: P19525 Human
- Unigene: 131431 Human
-
别名
- Double stranded RNA activated protein kinase; antibody
- E2AK2_HUMAN antibody
- eIF-2A protein kinase 2 antibody
see all
图片
-
All lanes : Anti-PKR (phospho T446) antibody [E120] (ab32036) at 1/2000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma) treated with 100 ng/mL Calyculin A whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma) treated with 100 ng/mL Calyculin A followed by 20 ng/mL TNF alpha whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Diluting buffer: 5% NFDM/TBST
Positive control: Rabbit monoclonal [Y117] to PKR (ab32506).
-
All lanes : Anti-PKR (phospho T446) antibody [E120] (ab32036) at 1/1000 dilution (unpurified)
Lane 1 : Hela (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Hela cell lysate + IFN + CalyculinA
Lane 3 : Hela cell lysate + Pptase
Predicted band size: 62 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted? -
PKR was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A(100nM 30min) whole cell lysate 10 µg with ab32036 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A(100nM 30min) whole cell lysate 10 µg
Lane 2: ab32036 IP in HeLa treated with Calyculin A(100nM 30min) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab32036 in HeLa treated with Calyculin A(100nM 30min) whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Lower bands could be cleavage form. (PMID:28702377)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (107)
ab32036 被引用在 107 文献中.
- Price AM et al. Adenovirus prevents dsRNA formation by promoting efficient splicing of viral RNA. Nucleic Acids Res 50:1201-1220 (2022). PubMed: 34671803
- Breuer J et al. What goes around comes around: Artificial circular RNAs bypass cellular antiviral responses. Mol Ther Nucleic Acids 28:623-635 (2022). PubMed: 35497503
- Lin WY et al. Repositioning of the Angiotensin II Receptor Antagonist Candesartan as an Anti-Inflammatory Agent With NLRP3 Inflammasome Inhibitory Activity. Front Immunol 13:870627 (2022). PubMed: 35669789
- Eo H & Valentine RJ Saturated Fatty Acid-Induced Endoplasmic Reticulum Stress and Insulin Resistance Are Prevented by Imoxin in C2C12 Myotubes. Front Physiol 13:842819 (2022). PubMed: 35936891
- Chludzinski E et al. Phenotypic and Transcriptional Changes of Pulmonary Immune Responses in Dogs Following Canine Distemper Virus Infection. Int J Mol Sci 23:N/A (2022). PubMed: 36077417