Anti-PKR抗体[YE350] (ab32052)


  • 产品名称Anti-PKR抗体[YE350]
    参阅全部 PKR 一抗
  • 描述
    兔单克隆抗体[YE350] to PKR
  • 特异性ab32052 recognises Protein kinase R (PKR). It does not cross-react with other GCN2 family members.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PKR aa 50-150.

  • 阳性对照
    • HeLa cell lysate and cells and human stomach carcinoma.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号YE350
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab32052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
IHC-P 1/100.
ICC/IF 1/100 - 1/500.
IP 1/20 - 1/100.


  • 功能Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Double stranded RNA activated protein kinase; antibody
    • E2AK2_HUMAN antibody
    • eIF-2A protein kinase 2 antibody
    • EIF2AK1 antibody
    • EIF2AK2 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
    • HGNC:9437 antibody
    • Interferon induced double stranded RNA activated protein kinase antibody
    • Interferon inducible elF2 alpha kinase antibody
    • Interferon inducible RNA dependent protein kinase antibody
    • Interferon-induced, double-stranded RNA-activated protein kinase antibody
    • Interferon-inducible RNA-dependent protein kinase antibody
    • MGC126524 antibody
    • P1/eIF-2A protein kinase antibody
    • P1/eIF2A protein kinase antibody
    • p68 kinase antibody
    • PKR antibody
    • PPP1R83 antibody
    • PRKR antibody
    • Protein kinase interferon inducible double stranded RNA dependent antibody
    • Protein kinase RNA activated antibody
    • Protein kinase RNA-activated antibody
    • Protein phosphatase 1 regulatory subunit 83 antibody
    • Serine/threonine protein kinase TIK antibody
    • Tyrosine protein kinase EIF2AK2 antibody
    see all

Anti-PKR antibody [YE350] 图像

  • Anti-PKR antibody [YE350] (ab32052) at 1/10000 dilution (purified) + MCF-7 whole cell lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 62 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
  • Anti-PKR antibody [YE350] (ab32052) at 1/10000 dilution (purified) + HeLa whole cell lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 62 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence staining of HeLa cells with purified ab32052 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32052 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemical staining of paraffin embedded human liver with purified ab32052 at a working dilution of 1/100. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-PKR antibody [YE350] (ab32052) at 1/5000 dilution (Unpurified) + HeLa cell lysate

    Predicted band size : 62 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using unpurified ab32052 at 1/250 dilution.

  • Immunofluorescent staining of HeLa cells using unpurified ab32052 at 1/250 dilution.

Anti-PKR antibody [YE350] (ab32052)参考文献

This product has been referenced in:
  • Atkinson NJ  et al. The influence of CpG and UpA dinucleotide frequencies on RNA virus replication and characterization of the innate cellular pathways underlying virus attenuation and enhanced replication. Nucleic Acids Res 42:4527-45 (2014). WB ; Human . Read more (PubMed: 24470146) »
  • Nejepinska J  et al. Reporters transiently transfected into mammalian cells are highly sensitive to translational repression induced by dsRNA expression. PLoS One 9:e87517 (2014). WB ; Human . Read more (PubMed: 24475301) »

See all 8 Publications for this product

Product Wall

Thank you for your enquiry regardingab32052 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cell line)
Loading amount 20 µg
Specification HeLa cell line
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Mirjam Eckert

Verified customer

提交于 Mar 26 2009