概述

  • 产品名称Anti-PKN2抗体[E352]
    参阅全部 PKN2 一抗
  • 描述
    兔单克隆抗体[E352] to PKN2
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near N-terminus of human PKN2

  • 阳性对照
    • 293T cell lysate human colon carcinoma
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号E352
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab32395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/250 - 1/500.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

靶标

  • 功能Exhibits a preference for highly basic protein substrates.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 1 protein kinase domain.
    Contains 3 REM (Hr1) repeats.
  • 结构域The C1 domain does not bind the diacylglycerol (DAG).
  • 翻译后修饰Autophosphorylated.
    Activated by limited proteolysis with trypsin.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cardiolipin activated protein kinase Pak2 antibody
    • MGC150606 antibody
    • MGC71074 antibody
    • PAK 2 antibody
    • PAK2 antibody
    • PKN gamma antibody
    • PKN2 antibody
    • PKN2_HUMAN antibody
    • PRK2 antibody
    • PRKCL2 antibody
    • PRO2042 antibody
    • Protein kinase C like 2 antibody
    • Protein kinase C related kinase 2 antibody
    • Protein kinase C-like 2 antibody
    • Protein kinase N2 antibody
    • Protein-kinase C-related kinase 2 antibody
    • Serine/threonine-protein kinase N2 antibody
    see all

Anti-PKN2 antibody [E352] 图像

  • Anti-PKN2 antibody [E352] (ab32395) at 1/1000 dilution + 10ug 293T cell lysate

    Predicted band size : 112 kDa
    Observed band size : 112 kDa
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ab32395 to stain PKN2

Anti-PKN2 antibody [E352] (ab32395)参考文献

This product has been referenced in:
  • Sun L  et al. Characterization of the interaction of Aha1 with components of the Hsp90 chaperone machine and client proteins. Biochim Biophys Acta 1823:1092-101 (2012). Read more (PubMed: 22504172) »
  • McPhee JB  et al. Delineation of regions of the Yersinia YopM protein required for interaction with the RSK1 and PRK2 host kinases and their requirement for interleukin-10 production and virulence. Infect Immun 78:3529-39 (2010). WB . Read more (PubMed: 20515922) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1 line)
Loading amount 10000 cells
Specification THP-1 line
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Feb 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"