Anti-PKN1抗体[1B10] (ab57424)


  • 产品名称Anti-PKN1抗体[1B10]
    参阅全部 PKN1 一抗
  • 描述
    小鼠单克隆抗体[1B10] to PKN1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human PKN1 aa 462-615.
    Database link: Q16512



Our Abpromise guarantee covers the use of ab57424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


  • 功能PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
  • 组织特异性Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 1 protein kinase domain.
    Contains 3 REM (Hr1) repeats.
  • 结构域The C1 domain does not bind the diacylglycerol (DAG).
  • 翻译后修饰Autophosphorylated; preferably on serine. Phosphorylated during mitosis.
    Activated by limited proteolysis with trypsin.
  • 细胞定位Cytoplasm. Nucleus. Endosome. Cell membrane. Cleavage furrow. Midbody. Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-374 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DBK antibody
    • PAK 1 antibody
    • PAK-1 antibody
    • PAK1 antibody
    • PKC1 antibody
    • PKN ALPHA antibody
    • PKN antibody
    • Pkn1 antibody
    • PKN1_HUMAN antibody
    • PRK1 antibody
    • PRKCL1 antibody
    • Protease activated kinase 1 antibody
    • Protease-activated kinase 1 antibody
    • Protein kinase C like 1 antibody
    • Protein kinase C like PKN antibody
    • Protein kinase C related kinase 1 antibody
    • Protein kinase C-like 1 antibody
    • Protein kinase C-like PKN antibody
    • Protein kinase N1 antibody
    • Protein kinase PKN alpha antibody
    • Protein kinase PKN-alpha antibody
    • Protein-kinase C-related kinase 1 antibody
    • Serine threonine kinase N antibody
    • Serine threonine protein kinase N antibody
    • Serine-threonine protein kinase N antibody
    • Serine/threonine protein kinase N1 antibody
    • Serine/threonine-protein kinase N1 antibody
    see all

Anti-PKN1 antibody [1B10] 图像

  • Western blot against tagged recombinant protein immunogen using ab57424 PKN1 antibody at 1ug/ml. Predicted band size of immunogen is 40 kDa
  • Immunofluorescence of ab57424 on HeLa cell at 10 ug/ml.

  • Immunoprecipitation of PKN1 transfected lysate using ab57424 and protein A magnetic bead

Anti-PKN1 antibody [1B10] (ab57424)参考文献

ab57424 has not yet been referenced specifically in any publications.

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