概述

  • 产品名称Anti-PKM2抗体[AT4M3]
    参阅全部 PKM2 一抗
  • 描述
    小鼠单克隆抗体[AT4M3] to PKM2
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length Human PKM2 (1-531aa) purified from E.coli (NP_002645).

  • 阳性对照
    • NIH3T3 and Jurkat cell lysates; HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab100841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/500. Predicted molecular weight: 58 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF 1/250 - 1/500.

靶标

  • 功能Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • 组织特异性Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • 序列相似性Belongs to the pyruvate kinase family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • 细胞定位Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CTHBP antibody
    • Cytosolic thyroid hormone binding protein antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • MGC3932 antibody
    • OIP 3 antibody
    • OIP-3 antibody
    • OIP3 antibody
    • OPA interacting protein 3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • PK muscle type antibody
    • PK, muscle type antibody
    • PK2 antibody
    • PK3 antibody
    • PKM antibody
    • PKM2 antibody
    • pykm antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase 3 antibody
    • Pyruvate kinase isozymes M1/M2 antibody
    • Pyruvate kinase muscle antibody
    • Pyruvate kinase muscle isozyme antibody
    • pyruvate kinase PKM antibody
    • Pyruvate kinase, muscle 2 antibody
    • TCB antibody
    • THBP1 antibody
    • Thyroid hormone binding protein 1 antibody
    • Thyroid hormone binding protein cytosolic antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2 PK antibody
    • Tumor M2-PK antibody
    see all

Anti-PKM2 antibody [AT4M3] 图像

  • All lanes : Anti-PKM2 antibody [AT4M3] (ab100841) at 1/500 dilution

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    HRP conjugated goat anti-mouse
    Developed using the ECL technique

    Predicted band size : 58 kDa
  • Immunofluorescence analysis of Human HeLa cells stained with ab100841 at 1/500 dilution with Texas Red (Red). Nuclear staining with Hoechst 3342 (Blue).

Anti-PKM2 antibody [AT4M3] (ab100841)参考文献

This product has been referenced in:
  • Hamabe A  et al. Role of pyruvate kinase M2 in transcriptional regulation leading to epithelial-mesenchymal transition. Proc Natl Acad Sci U S A 111:15526-31 (2014). Read more (PubMed: 25313085) »
  • Busch T  et al. Keratin 8 phosphorylation regulates keratin reorganization and migration of epithelial tumor cells. J Cell Sci 125:2148-59 (2012). Read more (PubMed: 22344252) »

See all 2 Publications for this product

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