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Synthetic peptide corresponding to Human PKM2 aa 476-505 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: 5315
There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. ab38237 recognizes the M1/M2 isoform of pyruvate kinase.
Our Abpromise guarantee covers the use of ab38237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/50 - 1/100.|
|WB||1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling PKM2 with ab38237. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PKM2 with ab38237 at 1/50. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by ACE staining.
Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling PKM2 (green) with ab38237. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.2%, 30 min). Cells were incubated with the primary antibody (1/200, 2 h at room temperature). An Alexa Fluor® 488-conjugated donkey anti-rabbit antibody was used as the secondary antibody (1/1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
ICC/IF image of ab38237 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38237, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.