概述
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产品名称Anti-PKLR抗体
参阅全部 PKLR 一抗 -
描述兔多克隆抗体to PKLR
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宿主Rabbit
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经测试应用适用于: WB, ICC/IFmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Cow
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免疫原
Recombinant fragment, corresponding to a region within the N terminal amino acids 1-230 of Human PKLR.
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阳性对照
- 293T, A431, H1299, HeLa, HepG2, Raji cell lysates
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab96604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 62 kDa. | |
| ICC/IF | 1/100 - 1/200. |
靶标
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功能Plays a key role in glycolysis.
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通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
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疾病相关Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia. -
序列相似性Belongs to the pyruvate kinase family.
- Information by UniProt
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数据库链接
- Entrez Gene: 539579 Cow
- Entrez Gene: 5313 Human
- Entrez Gene: 18770 Mouse
- Entrez Gene: 24651 Rat
- Omim: 609712 Human
- SwissProt: P30613 Human
- SwissProt: P53657 Mouse
- SwissProt: P12928 Rat
see all -
别名
- EC 2.7.1.40 antibody
- KPYR_HUMAN antibody
- L-PK antibody
see all
图片
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Western blot - Anti-PKLR antibody (ab96604)All lanes : Anti-PKLR antibody (ab96604) at 1/5000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 62 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PKLR antibody (ab96604)Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab96604 at 1:200 dilution. Image 2: Merged with DNA probe.
文献
ab96604 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"