概述

  • 产品名称Anti-PKLR抗体
    参阅全部 PKLR 一抗
  • 描述
    小鼠多克隆抗体to PKLR
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human PKLR protein, amino acids 1-574 (NP_000289.1)

  • 阳性对照
    • Human liver tissue lysate, PKLR transfected 293T cell lysate, Human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab89071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is recommended: Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Plays a key role in glycolysis.
    • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
    • 疾病相关Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
      Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
    • 序列相似性Belongs to the pyruvate kinase family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EC 2.7.1.40 antibody
      • KPYR_HUMAN antibody
      • L-PK antibody
      • Pk-1 antibody
      • PK1 antibody
      • PKL antibody
      • Pklg antibody
      • Pklr antibody
      • PKR antibody
      • PKRL antibody
      • Pyruvate kinase 1 antibody
      • Pyruvate kinase isozymes R/L antibody
      • Pyruvate kinase liver and blood cell antibody
      • Pyruvate kinase liver and RBC antibody
      • Pyruvate kinase liver and red blood cell antibody
      • Pyruvate kinase liver type antibody
      • Pyruvate kinase type L antibody
      • Pyruvate kinase, red cell type antibody
      • R type/L type pyruvate kinase antibody
      • R-PK antibody
      • R-type/L-type pyruvate kinase antibody
      • Red cell/liver pyruvate kinase antibody
      • RPK antibody
      see all

    Anti-PKLR antibody 图像

    • Anti-PKLR antibody (ab89071) at 1 µg/ml + Human liver tissue lysate at 50 µg

      Predicted band size : 62 kDa
      Observed band size : 62 kDa
    • All lanes : Anti-PKLR antibody (ab89071) at 1 µg/ml

      Lane 1 : PKLR transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 62 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
    • ab89071 at 3µg/ml staining PKLR in Human kidney by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

    Anti-PKLR antibody (ab89071)参考文献

    ab89071 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab89071.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"