概述

  • 产品名称Anti-PKLR抗体
    参阅全部 PKLR 一抗
  • 描述
    兔多克隆抗体to PKLR
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    SSLQLRSWVSKSQRD

    (Human) conjugated to KLH

  • 阳性对照
    • Breast carcinoma tissue and NIH/3T3 cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab38240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/1000.

靶标

  • 功能Plays a key role in glycolysis.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • 疾病相关Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
    Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
  • 序列相似性Belongs to the pyruvate kinase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.7.1.40 antibody
    • KPYR_HUMAN antibody
    • L-PK antibody
    • Pk-1 antibody
    • PK1 antibody
    • PKL antibody
    • Pklg antibody
    • Pklr antibody
    • PKR antibody
    • PKRL antibody
    • Pyruvate kinase 1 antibody
    • Pyruvate kinase isozymes R/L antibody
    • Pyruvate kinase liver and blood cell antibody
    • Pyruvate kinase liver and RBC antibody
    • Pyruvate kinase liver and red blood cell antibody
    • Pyruvate kinase liver type antibody
    • Pyruvate kinase type L antibody
    • Pyruvate kinase, red cell type antibody
    • R type/L type pyruvate kinase antibody
    • R-PK antibody
    • R-type/L-type pyruvate kinase antibody
    • Red cell/liver pyruvate kinase antibody
    • RPK antibody
    see all

Anti-PKLR antibody 图像

  • Anti-PKLR antibody (ab38240) at 1/100 dilution + NIH/3T3 cell lysate

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
    Additional bands at : 30 kDa,50 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human breast cancer tissue staineded with ab38240 at a 1/50 dilution, using a peroxidase-conjugated secondary antibody, followed by AEC staining.

    This data demonstrates the suitability of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-PKLR antibody (ab38240)参考文献

This product has been referenced in:
  • Li YG  et al. 1-deoxynojirimycin inhibits glucose absorption and accelerates glucose metabolism in streptozotocin-induced diabetic mice. Sci Rep 3:1377 (2013). WB ; Mouse . Read more (PubMed: 23536174) »
  • Li YG  et al. Cooperative anti-diabetic effects of deoxynojirimycin-polysaccharide by inhibiting glucose absorption and modulating glucose metabolism in streptozotocin-induced diabetic mice. PLoS One 8:e65892 (2013). WB ; Mouse . Read more (PubMed: 23755289) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. In answer to your question the sequence of the immunogen used to raise this antibody was SSLQLRSWVSKSQRD. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"