概述

  • 产品名称
  • 描述
    兔多克隆抗体to PKLR
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 178-510 of Human PKLR.(Uniprot:P30613)

  • 阳性对照
    • A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, HTC116 cell lysate and Human breast cancer tissue.

性能

应用

Our Abpromise guarantee covers the use of ab125697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 62 kDa.
IHC-P 1/100 - 1/500. Perfrom antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer(pH8.0).
ICC/IF 1/100 - 1/500.

靶标

  • 功能
    Plays a key role in glycolysis.
  • 通路
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • 疾病相关
    Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
    Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
  • 序列相似性
    Belongs to the pyruvate kinase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.7.1.40 antibody
    • KPYR_HUMAN antibody
    • L-PK antibody
    • Pk-1 antibody
    • PK1 antibody
    • PKL antibody
    • Pklg antibody
    • Pklr antibody
    • PKR antibody
    • PKRL antibody
    • Pyruvate kinase 1 antibody
    • Pyruvate kinase isozymes R/L antibody
    • Pyruvate kinase liver and blood cell antibody
    • Pyruvate kinase liver and RBC antibody
    • Pyruvate kinase liver and red blood cell antibody
    • Pyruvate kinase liver type antibody
    • Pyruvate kinase type L antibody
    • Pyruvate kinase, red cell type antibody
    • R type/L type pyruvate kinase antibody
    • R-PK antibody
    • R-type/L-type pyruvate kinase antibody
    • Red cell/liver pyruvate kinase antibody
    • RPK antibody
    see all

图片

  • ab125697, at 1/250 dilution, staining PKLR in Formalin-fixed, Paraffin-embedded Human breast cancer tissue by Immunohistochemistry.
  • ab125697, at 1/500 dilution, staining PKLR in Methanol-fixed HeLa cells by Immunocytochemistry/Immunofluorescence.
  • Anti-PKLR antibody (ab125697) at 1/1000 dilution + A549 cell lysate at 30 µg

    Predicted band size : 62 kDa

文献

ab125697 has not yet been referenced specifically in any publications.

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