Anti-PKLR抗体(ab125697)
Key features and details
- Rabbit polyclonal to PKLR
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PKLR抗体
参阅全部 PKLR 一抗 -
描述
兔多克隆抗体to PKLR -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment containing a sequence corresponding to a region within amino acids 178-510 of Human PKLR.(Uniprot:P30613)
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阳性对照
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125697于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 62 kDa.
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IHC-P |
1/100 - 1/500. Perfrom antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer(pH8.0).
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ICC/IF |
1/100 - 1/500.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 62 kDa. |
IHC-P
1/100 - 1/500. Perfrom antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer(pH8.0). |
ICC/IF
1/100 - 1/500. |
靶标
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功能
Plays a key role in glycolysis. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
疾病相关
Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia. -
序列相似性
Belongs to the pyruvate kinase family. - Information by UniProt
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数据库链接
- Entrez Gene: 5313 Human
- Omim: 609712 Human
- SwissProt: P30613 Human
- Unigene: 95990 Human
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别名
- EC 2.7.1.40 antibody
- KPYR_HUMAN antibody
- L-PK antibody
see all
图片
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Anti-PKLR antibody (ab125697) at 1/1000 dilution + A549 cell lysate at 30 µg
Predicted band size: 62 kDa -
ab125697, at 1/250 dilution, staining PKLR in Formalin-fixed, Paraffin-embedded Human breast cancer tissue by Immunohistochemistry.
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ab125697, at 1/500 dilution, staining PKLR in Methanol-fixed HeLa cells by Immunocytochemistry/Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125697 尚未被引用在任何文献中。