antigen sequence: CQDHLYNSLG RKGISAKSQP YHRSQSSSSV LINKSMDSIN YPSDVGKQQL LSLHRSSRCE SHQDLLPDIA DSHQQGTEKL SDLTLQDSQK VVVVN, corresponding to internal sequence amino acids 1158-1252 of Human PKHG1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- ARHGEF41 antibody
- PKHG1_HUMAN antibody
- Pleckstrin homology domain containing family G (with RhoGef domain) member 1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-PKHG1 antibody (ab121938)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab121938 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKHG1 antibody (ab121938)
ab121938, at 1/50, staining PKHG1 in paraffin-embedded Human rectum tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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