Anti-PKC zeta (phospho T560)抗体[EP2037AY] (ab62372)


  • 产品名称Anti-PKC zeta (phospho T560)抗体[EP2037AY]
    参阅全部 PKC zeta 一抗
  • 描述
    兔单克隆抗体[EP2037AY] to PKC zeta (phospho T560)
  • 特异性ab62372 detects PKC zeta phosphorylated at Threonine 560.
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human PKC zeta aa 550-650 (phospho T560). The exact sequence is proprietary.

  • 阳性对照
    • WB: HeLa cell lysates. IHC-P: Human brain tissue and human breast ductal carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EP2037AY
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab62372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/100.
  • 应用说明Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
    • 组织特异性Expressed in brain, and to a lesser extent in lung, kidney and testis.
    • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 OPR domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • 结构域The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
      The C1 domain does not bind the diacylglycerol (DAG).
    • 翻译后修饰CDH5 is required for its phosphorylation at Thr-410.
    • 细胞定位Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 14-3-3-zetaisoform antibody
      • AI098070 antibody
      • aPKCzeta antibody
      • C80388 antibody
      • EC antibody
      • KPCZ_HUMAN antibody
      • nPKC zeta antibody
      • nPKC-zeta antibody
      • OTTHUMP00000001368 antibody
      • OTTHUMP00000044160 antibody
      • PKC 2 antibody
      • PKC ZETA antibody
      • PKC2 antibody
      • Pkcz antibody
      • PKCZETA antibody
      • PKM-zeta, included antibody
      • PRKCZ antibody
      • Protein kinase C zeta antibody
      • Protein kinase C zeta form antibody
      • Protein kinase C zeta type antibody
      • r14-3-3 antibody
      • R74924 antibody
      • zetaPKC antibody
      see all

    Anti-PKC zeta (phospho T560) antibody [EP2037AY] 图像

    • All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372) at 1/20000 dilution

      Lane 1 : HeLa cell lysate - treated with Calyculin A
      Lane 2 : HeLa cell lysate - treated with Calyculin A and lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 68 kDa

      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of PKC zeta (pT560) peptide (Lane 1) and PKC zeta non-phospho peptide (Lane 2) labelling PKC zeta (pT560) with ab62372 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

      Lane 1 : Hela Cell Lysate (untreated with Calyculin A)
      Lane 2 : Hela Cell Lysate (Treated with Calyculin A)

      Predicted band size : 68 kDa
    • ab62372 at 1/100 dilution staining PKC zeta in human brain by Immunohistochemistry, Paraffin-embedded tissue.
    • ab62372 at 1/100 dilution staining PKC zeta in human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)参考文献

    This product has been referenced in:
    • Kusne Y  et al. Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFa in glioblastoma. Sci Signal 7:ra75 (2014). WB . Read more (PubMed: 25118327) »
    • Sakaguchi M  et al. DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for advanced glycation end products (RAGE) and modulates the RAGE-triggered signaling pathway in human keratinocytes. J Biol Chem 289:23389-402 (2014). Read more (PubMed: 25002577) »

    See all 4 Publications for this product

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    It appears that this image is an error. I have attached the correct image of a WB representing the appropriate molecular weight. The datasheet on our website will be updated shortly.

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