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RabMAb

Anti-PKC zeta (phospho T560)抗体[EP2037AY] (ab62372)

概述

  • 产品名称
    Anti-PKC zeta (phospho T560)抗体[EP2037AY]
    参阅全部 PKC zeta 一抗
  • 描述
    兔单克隆抗体[EP2037AY] to PKC zeta (phospho T560)
  • 特异性
    ab62372 detects PKC zeta phosphorylated at Threonine 560.
  • 经测试应用
    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human PKC zeta aa 550-650 (phospho T560). The exact sequence is proprietary.

  • 阳性对照
    • WB: HeLa cell lysates. IHC-P: Human brain tissue and human breast ductal carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EP2037AY
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能
      This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
    • 组织特异性
      Expressed in brain, and to a lesser extent in lung, kidney and testis.
    • 序列相似性
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 OPR domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • 结构域
      The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
      The C1 domain does not bind the diacylglycerol (DAG).
    • 翻译后修饰
      CDH5 is required for its phosphorylation at Thr-410.
    • 细胞定位
      Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 14-3-3-zetaisoform antibody
      • AI098070 antibody
      • aPKCzeta antibody
      • C80388 antibody
      • EC 2.7.11.13 antibody
      • KPCZ_HUMAN antibody
      • nPKC zeta antibody
      • nPKC-zeta antibody
      • OTTHUMP00000001368 antibody
      • OTTHUMP00000044160 antibody
      • PKC 2 antibody
      • PKC ZETA antibody
      • PKC2 antibody
      • Pkcz antibody
      • PKCZETA antibody
      • PKM-zeta, included antibody
      • PRKCZ antibody
      • Protein kinase C zeta antibody
      • Protein kinase C zeta form antibody
      • Protein kinase C zeta type antibody
      • r14-3-3 antibody
      • R74924 antibody
      • zetaPKC antibody
      see all

    Anti-PKC zeta (phospho T560) antibody [EP2037AY] 图像

    • All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372) at 1/20000 dilution

      Lane 1 : HeLa cell lysate - treated with Calyculin A
      Lane 2 : HeLa cell lysate - treated with Calyculin A and lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 68 kDa


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    • ab62372 at 1/100 dilution staining PKC zeta in human brain by Immunohistochemistry, Paraffin-embedded tissue.
    • Dot blot analysis of PKC zeta (pT560) peptide (Lane 1) and PKC zeta non-phospho peptide (Lane 2) labelling PKC zeta (pT560) with ab62372 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

      Lane 1 : Hela Cell Lysate (untreated with Calyculin A)
      Lane 2 : Hela Cell Lysate (Treated with Calyculin A)


      Predicted band size : 68 kDa
    • ab62372 at 1/100 dilution staining PKC zeta in human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)参考文献

    This product has been referenced in:
    • Lahey KA  et al. Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function. PLoS One 12:e0180259 (2017). Read more (PubMed: 28671992) »
    • Kaku Y  et al. PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells. Sci Rep 7:4554 (2017). Read more (PubMed: 28674456) »

    See all 11 Publications for this product

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