Anti-PKC zeta (phospho T560)抗体(ab59412)

概述

  • 产品名称Anti-PKC zeta (phospho T560)抗体
    参阅全部 PKC zeta 一抗
  • 描述
    兔多克隆抗体to PKC zeta (phospho T560)
  • 特异性Detects endogenous levels of PKC? only when phosphorylated at threonine 560.
  • 经测试应用适用于: WB, ELISA, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human PKCζ around the phosphorylation site of threonine 560 (Q-L-TP-P-D).

  • 阳性对照
    • Brain tissue,COS7 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab59412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
IHC-Fr Use at an assay dependent concentration. PubMed: 23675446

靶标

  • 功能This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
  • 组织特异性Expressed in brain, and to a lesser extent in lung, kidney and testis.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • 结构域The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
    The C1 domain does not bind the diacylglycerol (DAG).
  • 翻译后修饰CDH5 is required for its phosphorylation at Thr-410.
  • 细胞定位Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 14-3-3-zetaisoform antibody
    • AI098070 antibody
    • aPKCzeta antibody
    • C80388 antibody
    • EC 2.7.11.13 antibody
    • KPCZ_HUMAN antibody
    • nPKC zeta antibody
    • nPKC-zeta antibody
    • OTTHUMP00000001368 antibody
    • OTTHUMP00000044160 antibody
    • PKC 2 antibody
    • PKC ZETA antibody
    • PKC2 antibody
    • Pkcz antibody
    • PKCZETA antibody
    • PKM-zeta, included antibody
    • PRKCZ antibody
    • Protein kinase C zeta antibody
    • Protein kinase C zeta form antibody
    • Protein kinase C zeta type antibody
    • r14-3-3 antibody
    • R74924 antibody
    • zetaPKC antibody
    see all

Anti-PKC zeta (phospho T560) antibody 图像

  • All lanes : Anti-PKC zeta (phospho T560) antibody (ab59412) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells treated with PMA (125ng/ml, 30mins)
    Lane 2 : Extracts from COS7 cells treated with PMA (125ng/ml, 30mins) with phosphopeptide


    Predicted band size : 68 kDa
    Observed band size : 68 kDa
  • Immunohistochemical analysis of paraffin embedded human brain gliomas using ab59412 at 1/50 dilution

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab59412 at 1/50 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.
  • ELISA showing that ab59412 (1:20000) detects endogenous levels of PKC zeta only when phosphorylated at Thr560.

Anti-PKC zeta (phospho T560) antibody (ab59412)参考文献

This product has been referenced in:

See all 3 Publications for this product

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