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Synthetic phosphopeptide derived from human PKCζ around the phosphorylation site of threonine 560 (Q-L-TP-P-D).
Our Abpromise guarantee covers the use of ab59412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
|IHC-P||1/50 - 1/100.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23675446|
Immunohistochemical analysis of paraffin embedded human brain gliomas using ab59412 at 1/50 dilution
ELISA showing that ab59412 (1:20000) detects endogenous levels of PKC zeta only when phosphorylated at Thr560.