使用敲除细胞株进行验证RabMAb

Anti-PKC zeta抗体[EP1490(2)] (ab108970)

概述

  • 产品名称Anti-PKC zeta抗体[EP1490(2)]
    参阅全部 PKC zeta 一抗
  • 描述
    兔单克隆抗体[EP1490(2)] to PKC zeta
  • 经测试应用适用于: ICC/IF, WBmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human PKC zeta (UniProt Q05513).

  • 阳性对照
    • 293T, HT-29 and fetal liver lysates
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式Liquid
  • 存��说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EP1490(2)
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab108970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/400.
WB 1/1000 - 1/10000. Predicted molecular weight: 74 kDa.
  • 应用说明Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
    • 组织特异性Expressed in brain, and to a lesser extent in lung, kidney and testis.
    • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 OPR domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • 结构域The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
      The C1 domain does not bind the diacylglycerol (DAG).
    • 翻译后修饰CDH5 is required for its phosphorylation at Thr-410.
    • 细胞定位Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 14-3-3-zetaisoform antibody
      • AI098070 antibody
      • aPKCzeta antibody
      • C80388 antibody
      • EC 2.7.11.13 antibody
      • KPCZ_HUMAN antibody
      • nPKC zeta antibody
      • nPKC-zeta antibody
      • OTTHUMP00000001368 antibody
      • OTTHUMP00000044160 antibody
      • PKC 2 antibody
      • PKC ZETA antibody
      • PKC2 antibody
      • Pkcz antibody
      • PKCZETA antibody
      • PKM-zeta, included antibody
      • PRKCZ antibody
      • Protein kinase C zeta antibody
      • Protein kinase C zeta form antibody
      • Protein kinase C zeta type antibody
      • r14-3-3 antibody
      • R74924 antibody
      • zetaPKC antibody
      see all

    Anti-PKC zeta antibody [EP1490(2)] 图像



    • Predicted band size : 74 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: PKC zeta knockout HAP1 cell lysate (20 µg)

      Lane 3: HT-29 cell lysate (20 µg)

      Lane 4: A431 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108970 observed at 74 kDa. Red - loading control, ab8226, observed at 42 kDa.
      ab108970 was shown to specifically react with PKC zeta when PKC zeta knockout samples were used. Wild-type and PKC zeta knockout samples were subjected to SDS-PAGE. ab108970 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • ab108970 staining PKC zeta in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/400 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

      See Abreview

    • All lanes : Anti-PKC zeta antibody [EP1490(2)] (ab108970) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : HT-29 lysate
      Lane 3 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 74 kDa
      Observed band size : 74 kDa

    Anti-PKC zeta antibody [EP1490(2)] (ab108970)参考文献

    ab108970 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization No
    Fixative Methanol
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Jan 23 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"