ab59364 detects endogenous levels of total PKC zeta protein. The immunogen used for this product shares 89% homology with PKC iota/lambda. Cross-reactivity with this protein has not been confirmed experimentally.
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
Expressed in brain, and to a lesser extent in lung, kidney and testis.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 OPR domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain.
The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B. The C1 domain does not bind the diacylglycerol (DAG).
CDH5 is required for its phosphorylation at Thr-410.
Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
PKC zeta was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to PKC zeta and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab59364.
Immunofluorescence analysis of NIH/3T3 cells using PKC zeta at dilution of 1:100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta antibody (ab59364)Image from Rivero R et al., Reproductive Biology and Endocrinology 2012, 10:17, 5 March 2012. Fig 1.; doi: 10.1186/1477-7827-10-17.
Immunohistochemical analysis of Human proliferative endometrial tissue, staining PKC zeta with ab59364.
Antigen retrieval was performed by heat mediation in a citrate buffer. Tissue was blocked with blocking solution before incubating with primary antibody (1/100) overnight at 4°C. Staining was detected using DAB.
ab59364, at a 1/50 dilution, staining humna PKC zeta in brain, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue, minus immunising peptide (left image) and plus immunising peptide (right image).
Rivero R et al. Protein expression of PKCZ (Protein Kinase C Zeta), Munc18c, and Syntaxin-4 in the insulin pathway in endometria of patients with polycystic ovary syndrome (PCOS). Reprod Biol Endocrinol10:17 (2012).
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Kumashiro N et al. Cellular mechanism of insulin resistance in nonalcoholic fatty liver disease. Proc Natl Acad Sci U S A108:16381-5 (2011).
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