Anti-PKC theta (phospho T538)抗体(ab63365)

概述

  • 产品名称Anti-PKC theta (phospho T538)抗体
    参阅全部 PKC theta 一抗
  • 描述
    兔多克隆抗体to PKC theta (phospho T538)
  • 特异性ab63365 detects PKC theta only when phosphorylated at threonine 538.
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human PKC theta around the phosphorylation site of threonine 538 (T-N-TP-F-C).

  • 阳性对照
    • Jurkat cell extracts and human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab63365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).

靶标

  • 功能This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 组织特异性Skeletal muscle, megakaryoblastic cells and platelets.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 结构域The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • 翻译后修饰Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all

Anti-PKC theta (phospho T538) antibody 图像

  • All lanes : Anti-PKC theta (phospho T538) antibody (ab63365) at 1/500 dilution

    Lane 1 : Jurkat cell extracts
    Lane 2 : Jurkat cell extracts with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 82 kDa
    Observed band size : 82 kDa
  • ab63365 at 1/50 dilution staining PKC theta in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Anti-PKC theta (phospho T538) antibody (ab63365)参考文献

ab63365 has not yet been referenced specifically in any publications.

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In this case I would suggest using acapture ELISA assay. Here, the capture antibody will be an antibodyto capture total PKC theta and thedetection antibody will be yourab63365. For the capture antibody y...

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