Anti-PKC theta (phospho S676)抗体(ab47774)


  • 产品名称
    Anti-PKC theta (phospho S676)抗体
    参阅全部 PKC theta 一抗
  • 描述
    兔多克隆抗体to PKC theta (phospho S676)
  • 特异性
    This antibody detects PKC theta only when phosphorylated at serine 676.
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human PKC theta aa 643-692 (C terminal).

  • 阳性对照
    • IHC-P: Human breast carcinoma tissue. WB: JK cell extracts.



Our Abpromise guarantee covers the use of ab47774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/4000.


  • 功能
    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 组织特异性
    Skeletal muscle, megakaryoblastic cells and platelets.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 结构域
    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • 翻译后修饰
    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all

Anti-PKC theta (phospho S676) antibody 图像

  • This image shows human breast carcinoma stained with ab47774 at 1/50 dilution. Right hand image shows tissue treated with the immunogenic phosphopeptide, whereas the left hand image is untreated.
  • All lanes : Anti-PKC theta (phospho S676) antibody (ab47774) at 1/500 dilution

    Lane 1 : JK cell extract treated with 200nM PMA for 30mins. No peptide.
    Lane 2 : JK cell extract, untreated. No peptide.

    Predicted band size : 82 kDa

Anti-PKC theta (phospho S676) antibody (ab47774)参考文献

This product has been referenced in:
  • Li J  et al. Nuclear PKC-? facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation. J Cell Sci 129:2448-61 (2016). WB ; Human . Read more (PubMed: 27149922) »
  • Aguiló JI  et al. Protein kinase C-theta is required for NK cell activation and in vivo control of tumor progression. J Immunol 182:1972-81 (2009). WB ; Mouse . Read more (PubMed: 19201850) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Tissue lysate - whole (LIVER)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Dec 06 2016

I did check with the lab regarding the protocol used, the cells should be serum starved before PMA treatment and the concentration is 200nM.