The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: assay dependent.
Immunohistochemistry: 1/250 (alkaline phosphatase or horseradish peroxidase).
Western blot (enhanced chemiluminescence: 1/250 (alkaline phosphatase or horseradish peroxidase). Predicted molecular weight: 85 kDa.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
With a more sensitive detection system, such as
enhanced chemiluminescence or ELISA, the antibody may be diluted further.
It has also been reported to be useful for
immunoprecipitation of PKC theta.
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Skeletal muscle, megakaryoblastic cells and platelets.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.