兔单克隆抗体[EP1493-62] to PKC mu (phospho S738 + S742)
A synthetic phospho-peptide corresponding to residues surrounding serine 738 and 742 of human PKC mu.
- HeLa cell lysate treated with TPA.
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/100 - 1/500. Predicted molecular weight: 102 kDa.
Is unsuitable for Flow Cyt,ICC or IHC-P.
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
Information by UniProt
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
Western blot - PKC mu (phospho S744 + S748) antibody [EP1439Y] (ab75878)
All lanes :
Anti-PKC mu (phospho S738 + S742) antibody [EP1493-62] (ab75878) at 1/500 dilutionLane 1 :
HeLa cell lysateLane 2 :
HeLa cell lysate treated with TPA
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat anti-rabbit HRP at 1/1000 dilutionPredicted band size:
102 kDaObserved band size:
115 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"