Anti-PKC mu (phospho S738 + S742)抗体(ab74105)

概述

  • 产品名称Anti-PKC mu (phospho S738 + S742)抗体
    参阅全部 PKC mu 一抗
  • 描述
    兔多克隆抗体to PKC mu (phospho S738 + S742)
  • 特异性ab74105 detects endogenous levels of PKC mu only when phosphorylated at serine 738 and serine 742.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthesized phosphopeptide derived from human PKC mu around the phosphorylation site of serine 738 and serine 742 (E-K-SP-F-R-R-SP-V-V).

  • 阳性对照
    • WB: extracts from A549 cells treated with PMA (125ng/ml, 30mins), and HT-29 cells treated with serum (20%, 15mins). IHC-P: human heart tissue.

性能

应用

Our Abpromise guarantee covers the use of ab74105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 102 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 细胞定位Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KPCD1_HUMAN antibody
    • nPKC D1 antibody
    • nPKC mu antibody
    • nPKC-D1 antibody
    • nPKC-mu antibody
    • nPKCD1 antibody
    • nPKCmu antibody
    • PKC antibody
    • PKC MU antibody
    • PKCM antibody
    • PKCmu antibody
    • PKD 1 antibody
    • PKD antibody
    • PKD1 antibody
    • PRKCM antibody
    • PRKD 1 antibody
    • Prkd1 antibody
    • Protein kinase C mu antibody
    • Protein kinase C mu type antibody
    • Protein kinase D antibody
    • Protein kinase D1 antibody
    • Serine/threonine protein kinase D1 antibody
    • Serine/threonine-protein kinase D1 antibody
    see all

Anti-PKC mu (phospho S738 + S742) antibody 图像

  • All lanes : Anti-PKC mu (phospho S738 + S742) antibody (ab74105) at 1/500 dilution

    Lane 1 : Extracts from A549 cells treated with PMA (125ng/ml, 30mins), plus phosphopeptide at 10 µg
    Lane 2 : Extracts from HT-29 cells treated with serum (20%, 15mins), plus phosphopeptide at 10 µg
    Lane 3 : Extracts from A549 cells treated with PMA (125ng/ml, 30mins), plus serum (20%, 15mins) and phopeptide at 10 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 102 kDa
    Observed band size : 102 kDa
  • ab74105, at a 1/50 dilution, staining PKC mu in paraffin embedded human heart tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.

Anti-PKC mu (phospho S738 + S742) antibody (ab74105)参考文献

This product has been referenced in:
  • Pearen MA  et al. The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue. Mol Endocrinol 26:372-84 (2012). WB ; Mouse . Read more (PubMed: 22282471) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Rat Cell lysate - whole cell (Astrocytes)
Specification Astrocytes
Treatment 10 nM ofControl, Insulin, IGF-1 or both hormones for 15 minutes
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Edwin Hernandez

Verified customer

提交于 May 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"