兔单克隆抗体[EP1493Y] to PKC mu
Mouse, Rat, Human
A synthetic peptide corresponding to residues within human PKC mu was used as an immunogen.
A trial size is available to purchase for this antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 102 kDa).
Is unsuitable for Flow Cyt, IHC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
Information by UniProt
nPKC D1 antibody
nPKC mu antibody
Western blot - Anti-PKC mu antibody [EP1493Y] (ab51246)
All lanes :
Anti-PKC mu antibody [EP1493Y] (ab51246) at 1/1000 dilution
Lane 1 :
HeLa cell lysate, untreated
Lane 2 :
HeLa cell lysate, treated with PMA
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size:
Observed band size:
105 kDa (
why is the actual band size different from the predicted?
This product has been referenced in:
et al. Trypanosoma cruzi extracellular amastigotes trigger the protein kinase D1-cortactin-actin pathway during cell invasion. Cell Microbiol 17:1797-810 (2015).
Read more (PubMed: 26096820) »
et al. Protein kinase D1 mediates stimulation of DNA synthesis and proliferation in intestinal epithelial IEC-18 cells and in mouse intestinal crypts. J Biol Chem 286:511-20 (2011).
Read more (PubMed: 21051537) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"