概述

  • 产品名称Anti-PKC iota抗体
    参阅全部 PKC iota 一抗
  • 描述
    小鼠单克隆抗体to PKC iota
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-588 of Human PKC iota

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab57422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. May play a role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. Functions in both pro- and anti-apoptotic pathways. Functions in the RAC1/ERK signaling required for transformed growth. Plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs).
    • 组织特异性Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers.
    • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 OPR domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • 结构域The OPR domain mediates interaction with SQSTM1.
      The C1 domain does not bind diacylglycerol (DAG).
    • 翻译后修饰On neuronal growth factor (NGF) stimulation, phosphorylated by Src on Tyr-265, Tyr-280 and Tyr-334. Phosphorylation on Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation.
    • 细胞定位Cytoplasm. Membrane. Endosome. Nucleus. Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesicular tubular clusters. Transported to VTCs through interaction with RAB2A.
    • Information by UniProt
    • 数据库链接
    • 别名
      • aPKC lambda/iota antibody
      • aPKC-lambda/iota antibody
      • Atypical protein kinase C lambda/iota antibody
      • Atypical protein kinase C-lambda/iota antibody
      • DXS1179E antibody
      • KPCI_HUMAN antibody
      • MGC26534 antibody
      • nPKC iota antibody
      • nPKC-iota antibody
      • nPKCiota antibody
      • PKC lambda antibody
      • PKC lambda/iota antibody
      • PKCI antibody
      • PKCiota antibody
      • PKClambda antibody
      • PRKC iota antibody
      • PRKC lambda/iota antibody
      • PRKC-lambda/iota antibody
      • PRKCI antibody
      • Protein kinase C iota antibody
      • Protein kinase C iota type antibody
      see all

    Anti-PKC iota antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab57422 PKC iota antibody at 1ug/ml. Predicted band size of immunogen is 91 kDa

    Anti-PKC iota antibody (ab57422)参考文献

    ab57422 has not yet been referenced specifically in any publications.

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