RabMAb

Anti-PKC gamma (phospho T514)抗体[EP2730Y] (ab109539)

概述

  • 产品名称Anti-PKC gamma (phospho T514)抗体[EP2730Y]
    参阅全部 PKC gamma 一抗
  • 描述
    兔单克隆抗体[EP2730Y] to PKC gamma (phospho T514)
  • 经测试应用适用于: Dot blot, WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding threonine 514 of Human PKC gamma.

  • 阳性对照
    • HeLa cell lysate; Human brain tissue; Sh-Sy-5Y cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • 组织特异性Expressed in Purkinje cells of the cerebellar cortex.
    • 疾病相关Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
    • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • KPCG_HUMAN antibody
      • MGC57564 antibody
      • OTTHUMP00000067291 antibody
      • PKC-gamma antibody
      • PKCC antibody
      • PKCG antibody
      • PRKCG antibody
      • Protein kinase C gamma antibody
      • Protein kinase C gamma polypeptide antibody
      • Protein kinase C gamma type antibody
      • Protein kinase C, gamma antibody
      • SCA 14 antibody
      • SCA14 antibody
      see all

    Anti-PKC gamma (phospho T514) antibody [EP2730Y] 图像

    • All lanes : Anti-PKC gamma (phospho T514) antibody [EP2730Y] (ab109539) at 0.02 µg/ml

      Lane 1 : HeLa cell lysate with PKC gamma (pT514) peptide
      Lane 2 : HeLa cell lysate with PKC gamma non-phospho peptide
      Lane 3 : HeLa cell lysate


      Predicted band size : 78 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)


      Exposure time : 15 seconds
    • All lanes : Anti-PKC gamma (phospho T514) antibody [EP2730Y] (ab109539) at 1/20000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 78 kDa
    • ab109539 at 1/100 dilution staining PKC gamma (phospho T514) in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • ab109539 at 1/100 dilution staining PKC gamma (phospho T514) in Sh-Sy-5Y cells by Immunofluorescence.
    • Dot blot analysis of PKC gamma (pT514) phospho peptide (Lane 1) and PKC gamma non-phospho peptide (Lane 2) using ab109539 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

      Blocking and Diluting buffer and concentration: 5% NFDM /TBST.

      Exposure time: 3 minutes.

    Anti-PKC gamma (phospho T514) antibody [EP2730Y] (ab109539)参考文献

    This product has been referenced in:
    • Huang L  et al. Ketamine potentiates hippocampal neurodegeneration and persistent learning and memory impairment through the PKC?-ERK signaling pathway in the developing brain. Brain Res 1476:164-71 (2012). WB . Read more (PubMed: 22985497) »
    • Dunn EF & Connor JH Dominant inhibition of Akt/protein kinase B signaling by the matrix protein of a negative-strand RNA virus. J Virol 85:422-31 (2011). Read more (PubMed: 20980511) »

    See all 2 Publications for this product

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