1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/250.
应用说明Is unsuitable for Flow Cyt.
功能This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
组织特异性Expressed in Purkinje cells of the cerebellar cortex.
疾病相关Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.
ab109539 at 1/100 dilution staining PKC gamma (phospho T514) in Sh-Sy-5Y cells by Immunofluorescence.
Dot blot - Anti-PKC gamma (phospho T514) antibody [EP2730Y] (ab109539)
Dot blot analysis of PKC gamma (pT514) phospho peptide (Lane 1) and PKC gamma non-phospho peptide (Lane 2) using ab109539 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.
Blocking and Diluting buffer and concentration: 5% NFDM /TBST.
Huang L et al. Ketamine potentiates hippocampal neurodegeneration and persistent learning and memory impairment through the PKC?-ERK signaling pathway in the developing brain. Brain Res1476:164-71 (2012).
Read more (PubMed: 22985497) »
Dunn EF & Connor JH Dominant inhibition of Akt/protein kinase B signaling by the matrix protein of a negative-strand RNA virus. J Virol85:422-31 (2011).
Read more (PubMed: 20980511) »