Anti-PKC gamma抗体(ab4144)

概述

  • 产品名称
    Anti-PKC gamma抗体
    参阅全部 PKC gamma 一抗
  • 描述
    兔多克隆抗体to PKC gamma
  • 经测试应用
    适用于: ICC/IF, ELISA, IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mammal
  • 免疫原

    Synthetic peptide:

    FEACNYPLELYEVSRYGPSSS

    , corresponding to amino acids 302-322 of Human PKC gamma.

性能

应用

Our Abpromise guarantee covers the use of ab4144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
ELISA
IHC-P
IHC-Fr
WB
  • 应用说明
    ELISA: assay dependent.
    Immunohistochemistry: 1/250 (using horseradish peroxidase or alkaline phosphatase).
    Western blot (using horseradish peroxidase or alkaline phosphatase): 1/250. Predicted molecular weight: 84 kDa.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.

    It has also been reported to be useful for immunoprecipitation of PKC gamma.
  • 靶标

    • 功能
      This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • 组织特异性
      Expressed in Purkinje cells of the cerebellar cortex.
    • 疾病相关
      Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
    • 序列相似性
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • KPCG_HUMAN antibody
      • MGC57564 antibody
      • OTTHUMP00000067291 antibody
      • PKC-gamma antibody
      • PKCC antibody
      • PKCG antibody
      • PRKCG antibody
      • Protein kinase C gamma antibody
      • Protein kinase C gamma polypeptide antibody
      • Protein kinase C gamma type antibody
      • Protein kinase C, gamma antibody
      • SCA 14 antibody
      • SCA14 antibody
      see all

    Anti-PKC gamma antibody 图像

    Anti-PKC gamma antibody (ab4144)参考文献

    ab4144 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HT1080 Cell line)
    Specification
    HT1080 Cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Username

    Abcam user community

    Verified customer

    提交于 Feb 17 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HT1080 Cell line)
    Loading amount
    20000 cells
    Specification
    HT1080 Cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 10 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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