Anti-PKC epsilon (phospho S729)抗体(ab63387)


  • 产品名称Anti-PKC epsilon (phospho S729)抗体
    参阅全部 PKC epsilon 一抗
  • 描述
    兔多克隆抗体to PKC epsilon (phospho S729)
  • 特异性Detects endogenous levels of PKC epsilon only when phosphorylated at serine 729.
  • 经测试应用适用于: ICC/IF, WB, ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Human
  • 免疫原

    Synthetic phosphopeptide from around the phosphorylation site of serine 729 (GFSPYF) of human PKC epsilon

  • 阳性对照
    • HeLa cells treated with PMA (125ng/ml, 30mins) Jurkat cells treated with PMA (125ng/ml, 30mins) NIH/3T3 cells treated with PMA (125ng/ml, 30mins) This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab63387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
ELISA 1/10000.
IP Use a concentration of 5 µg/ml.


  • 功能This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 结构域The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • 翻译后修饰Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KPCE_HUMAN antibody
    • MGC125656 antibody
    • MGC125657 antibody
    • nPKC epsilon antibody
    • nPKC-epsilon antibody
    • PKCE antibody
    • Pkcea antibody
    • PRKCE antibody
    • Protein kinase C epsilon antibody
    • Protein kinase C epsilon type antibody
    see all

Anti-PKC epsilon (phospho S729) antibody 图像

  • All lanes : Anti-PKC epsilon (phospho S729) antibody (ab63387) at 1/500 dilution

    Lane 1 : HeLa cells treated with PMA (125ng/ml, 30mins) at 30 µg
    Lane 2 : Jurkat cells treated eith PMA (125ng/ml, 30mins) at 30 µg
    Lane 3 : NIH/3T3 cells treated with PMA (125ng/ml, 30mins) at 30 µg
    Lane 4 : NIH/3T3 cells treated with syntesized peptide (125ng/ml, 30mins) at 10 µg

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
  • PKC epsilon was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to PKC epsilon and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab63387.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 84kDa; PKC epsilon
  • ICC/IF image of ab63387 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab63387 at 5µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PKC epsilon (phospho S729) antibody (ab63387)参考文献

This product has been referenced in:
  • Björnström K  et al. Orexin A inhibits propofol-induced neurite retraction by a phospholipase D/protein kinase Ce-dependent mechanism in neurons. PLoS One 9:e97129 (2014). WB ; Rat . Read more (PubMed: 24828410) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (10%)
Sample Guinea pig Cell lysate - other (cardiac myocyte)
Specification cardiac myocyte
Treatment 100nM drug1 for 30min, 1uM drug1 for 30min, 10uM drug1 for 30min, 100uM drug1 for 15min, 100uM drug1 for 30min, 100uM drug1 for 60min
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

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提交于 Jul 07 2014

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